CAJ | 학술논문

目的：探讨早期喉癌手术阴性切缘组织中PCNA、COX-2的表达与肿瘤局部复发的关系。方法选择63例经CO2激光手术治疗后的早期喉癌患者标本,对切除的肿瘤组织及切缘组织中PCNA、COX-2表达进行检测,所有患者均随访2年以上,对其生存及肿瘤复发情况进行观察。结果63例早期喉癌患者肿瘤组织中PCNA、COX-2阳性率分别为73．02%、71．43%；切缘组织中PCNA和COX-2阳性率分别为33．33%、30．16%。肿瘤组织与切缘组织中PCNA、COX-2均阳性的患者分别为41例和13例,阳性率分别为65．08%、20．63%；63例随访病例中,局部复发17例,复发率为26．98%。 PCNA阳性患者复发率为71.43%,COX-2阳性患者复发率为73．68%(P<0．05)。联合检测PCNA和COX-2阳性者复发率明显高于单独阳性者(P<0．05)。结论早期喉癌手术切缘组织中PCNA、COX-2的表达是喉癌患者预后的重要生物学标志,并可根据检查结果制定针对性的后续治疗方案,联合检测二者对术后治疗具有更可靠的指导意义。
목적：탐토조기후암수술음성절연조직중PCNA、COX-2적표체여종류국부복발적관계。방법선택63례경CO2격광수술치료후적조기후암환자표본,대절제적종류조직급절연조직중PCNA、COX-2표체진행검측,소유환자균수방2년이상,대기생존급종류복발정황진행관찰。결과63례조기후암환자종류조직중PCNA、COX-2양성솔분별위73．02%、71．43%；절연조직중PCNA화COX-2양성솔분별위33．33%、30．16%。종류조직여절연조직중PCNA、COX-2균양성적환자분별위41례화13례,양성솔분별위65．08%、20．63%；63례수방병례중,국부복발17례,복발솔위26．98%。 PCNA양성환자복발솔위71.43%,COX-2양성환자복발솔위73．68%(P<0．05)。연합검측PCNA화COX-2양성자복발솔명현고우단독양성자(P<0．05)。결론조기후암수술절연조직중PCNA、COX-2적표체시후암환자예후적중요생물학표지,병가근거검사결과제정침대성적후속치료방안,연합검측이자대술후치료구유경가고적지도의의。
Purpose To explore the relationship between expression of PCNA and COX-2 in the early laryngeal cancer with negative surgical margins and the local recurrence of tumor. Methods Totally 63 patients with early laryngeal cancer were enrolled in this stud-y, CO2 laser surgery was adopted as treatment, the expression of PCNA and COX-2 was detected in the resected tumor tissue and surgi-cal margins, and the survival and tumor recurrence were also observed. Results The positive rate of the expression of PCNA and COX-2 in the 63 patients with early laryngeal cancer tumor tissues were 73. 02% and 71. 43%, while that in the cutting edge were 33. 33% and 30. 16%, respectively. The expression of PCNA and COX-2 in tumor tissue and cutting edge were of all positive in 41 cases and 13 cases, and the positive rate was 65. 08% and 20. 63%, respectively. In the all followed-up patients, 17 cases were detected of local recurrence, the recurrence rate was 26. 98%, and the recurrence rate in the patients with PCNA positive was 71. 43%, while in the patients with COX-2 positive was 73. 68% (P<0. 05). The combined detection of PCNA and COX-2 positive recurrence rate was significantly higher than that of single positive (P<0. 05). Conclusion PCNA and COX-2 in the early laryngeal cancer with negative surgical margins are important biological markers for the evaluation of prognosis of laryngeal cancer patients, and to formulate the subse-quent treatment regimen. The combined detection of two proteins is more reliable for postoperative management.