临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2014年
4期
379-382
,共4页
结直肠肿瘤%Bmi-1%p14 ARF%Mdm2%免疫组织化学
結直腸腫瘤%Bmi-1%p14 ARF%Mdm2%免疫組織化學
결직장종류%Bmi-1%p14 ARF%Mdm2%면역조직화학
colorectal neoplasms%Bmi-1%p14 ARF%Mdm2%immunohistochemistry
目的：探讨Bmi-1、p14 ARF和Mdm2在结直肠癌中的表达及其与各临床病理参数的关系。方法应用免疫组化EliVision两步法检测125例结直肠癌和20例正常结直肠组织中Bmi-1、p14ARF和Mdm2的表达。结果(1)Bmi-1和Mdm2在结直肠癌中的阳性率(56.8%和62.4%)明显高于正常结直肠组织(20．0%和15.0%),p14ARF在结直肠癌中的阳性率(47．2%)明显低于正常结直肠组织(75．0%)(P<0．05)。(2)Bmi-1表达与浆膜侵犯、淋巴结转移、临床分期密切相关,p14ARF表达与浆膜侵犯、临床分期密切相关,Mdm2表达与组织分化程度、淋巴结转移、临床分期密切相关(P<0．05)。(3)在结直肠癌中,Bmi-1和Mdm2表达与p14 ARF表达均呈负相关( P<0．05)。结论 Bmi-1、p14 ARF和Mdm2的异常表达参与结直肠癌的发生、发展及转移,可作为评价结直肠癌生物学行为的参考指标,并为临床治疗提供新靶点。
目的：探討Bmi-1、p14 ARF和Mdm2在結直腸癌中的錶達及其與各臨床病理參數的關繫。方法應用免疫組化EliVision兩步法檢測125例結直腸癌和20例正常結直腸組織中Bmi-1、p14ARF和Mdm2的錶達。結果(1)Bmi-1和Mdm2在結直腸癌中的暘性率(56.8%和62.4%)明顯高于正常結直腸組織(20．0%和15.0%),p14ARF在結直腸癌中的暘性率(47．2%)明顯低于正常結直腸組織(75．0%)(P<0．05)。(2)Bmi-1錶達與漿膜侵犯、淋巴結轉移、臨床分期密切相關,p14ARF錶達與漿膜侵犯、臨床分期密切相關,Mdm2錶達與組織分化程度、淋巴結轉移、臨床分期密切相關(P<0．05)。(3)在結直腸癌中,Bmi-1和Mdm2錶達與p14 ARF錶達均呈負相關( P<0．05)。結論 Bmi-1、p14 ARF和Mdm2的異常錶達參與結直腸癌的髮生、髮展及轉移,可作為評價結直腸癌生物學行為的參攷指標,併為臨床治療提供新靶點。
목적：탐토Bmi-1、p14 ARF화Mdm2재결직장암중적표체급기여각림상병리삼수적관계。방법응용면역조화EliVision량보법검측125례결직장암화20례정상결직장조직중Bmi-1、p14ARF화Mdm2적표체。결과(1)Bmi-1화Mdm2재결직장암중적양성솔(56.8%화62.4%)명현고우정상결직장조직(20．0%화15.0%),p14ARF재결직장암중적양성솔(47．2%)명현저우정상결직장조직(75．0%)(P<0．05)。(2)Bmi-1표체여장막침범、림파결전이、림상분기밀절상관,p14ARF표체여장막침범、림상분기밀절상관,Mdm2표체여조직분화정도、림파결전이、림상분기밀절상관(P<0．05)。(3)재결직장암중,Bmi-1화Mdm2표체여p14 ARF표체균정부상관( P<0．05)。결론 Bmi-1、p14 ARF화Mdm2적이상표체삼여결직장암적발생、발전급전이,가작위평개결직장암생물학행위적삼고지표,병위림상치료제공신파점。
Purpose To explore the expression of Bmi-1, p14ARF and Mdm2 in colorectal carcinoma and to find out their association with clinicopathological features. Methods The expression of Bmi-1, p14ARF and Mdm2 was detected by immunohistochemistry in 125 cases of colorectal carcinoma and 20 cases of normal colorectal tissues. Results ( 1 ) The positive rates of Bmi-1 and Mdm2 were 56. 8% and 62. 4% in colorectal carcinoma, respectively, which were much higher than that in normal colorectal tissues (20. 0% and 15. 0%). The positive rates of p14ARF was 47. 2% in colorectal carcinoma, which was much lower than that 75. 0% in normal colorec-tal tissues ( P<0. 05 ) . ( 2 ) The expression of Bmi-1 was significantly correlated with serosal invasion, lymph node metastasis and Dukes stage. The expression of p14ARF was significantly correlated with serosal invasion and Dukes stage. The expression of Mdm2 was significantly correlated with histologic grade, lymph node metastasis and Dukes stage (P<0. 05). (3) In colorectal carcinoma, the expression of Bmi-1 and Mdm2 was negatively correlated with p14ARF, respectively (P<0. 05). Conclusions Abnormal expression of Bmi-1, p14ARF and Mdm2 is involved in the occurrence, development and metastasis of colorectal carcinoma, which could be as the reference factors in judgement of biological behavior and provide a new target for clinical treatment.
