CAJ | 학술논문

目的：探讨胃癌组织中RKIP基因启动子甲基化及蛋白的表达,分析RKIP基因启动子甲基化及蛋白表达与胃癌生物学行为的关系。方法采用甲基化特异性PCR( methylation-specific PCR, MSP)技术和免疫组化SP法检测45例胃癌组织、21例癌旁胃黏膜组织中RKIP基因启动子甲基化及蛋白的表达。结果胃癌及癌旁胃黏膜组织中RKIP基因启动子的甲基化阳性率分别为48．89%和4．8%(P<0．05)；RKIP蛋白在胃癌及癌旁胃黏膜组织中的阳性率分别为42．22%和90．48%(P<0．05)；RKIP基因启动子甲基化及蛋白的表达分别与胃癌的分化程度及淋巴结转移有关( P<0．05)。 RKIP基因启动子甲基化与蛋白表达呈明显负相关(P<0．05)。结论 RKIP基因启动子的异常甲基化是引起蛋白表达缺失的原因之一,RKIP蛋白表达缺失与胃癌的发生、发展有关。
목적：탐토위암조직중RKIP기인계동자갑기화급단백적표체,분석RKIP기인계동자갑기화급단백표체여위암생물학행위적관계。방법채용갑기화특이성PCR( methylation-specific PCR, MSP)기술화면역조화SP법검측45례위암조직、21례암방위점막조직중RKIP기인계동자갑기화급단백적표체。결과위암급암방위점막조직중RKIP기인계동자적갑기화양성솔분별위48．89%화4．8%(P<0．05)；RKIP단백재위암급암방위점막조직중적양성솔분별위42．22%화90．48%(P<0．05)；RKIP기인계동자갑기화급단백적표체분별여위암적분화정도급림파결전이유관( P<0．05)。 RKIP기인계동자갑기화여단백표체정명현부상관(P<0．05)。결론 RKIP기인계동자적이상갑기화시인기단백표체결실적원인지일,RKIP단백표체결실여위암적발생、발전유관。
Purpose To investigate the correlation between promoter methylation of RKIP and the clinicopathological features of gastric carcinoma and to analyze the correlation between promoter methylation of RKIP and expression of RKIP. Methods The protein ex-pression and promoter methylation of RKIP were detected in 45 cases of surgically resected gastric carcinoma specimens and 21 cases of corresponding normal tissues using the immunohistchemistry SP method and methylation-specific PCR ( MSP) . Results The positive rate of promoter methylation of RKIP was 48. 89% in gastric carcinoma tissues and 4. 8% in adjacent tissues (P<0. 05). The positive rate of the expression of RKIP protein was 42. 22% in gastric carcinoma tissues and 90. 48% in adjacent tissues (P<0. 05). The pro-tein expression and promoter methylation of RKIP were correlated with the degree of differentiation and lymph node metastasis ( P<0. 05) . Expression of RKIP protein in gastric carcinoma was associated with promoter methylation of RKIP. Conclusions Promoter abnormal methylation of RKIP may be one reason of the deletion of expression of RKIP protein, which correlates with occurrence and development of gastric carcinoma.