CAJ | 학술논문

目的：检测肿瘤干细胞标志物CD24在140例贲门腺癌中的表达,探讨其与腺癌临床病理学参数的关系。方法采用免疫组化SP法检测140例贲门腺癌中CD24的表达,分析其与临床病理学特征及生物学行为之间的相关性；收集新鲜癌组织与癌旁组织各26例,依据不同临床分期、不同分化程度进行分组,分别提取两组中的总RNA进行RT-PCR检测,计算CD24的相对表达量,分析两组间的差异性,进一步分析二者的侵袭性。结果(1)免疫组化检测结果：CD24在癌组织中的表达高于癌旁组织(P<0．05)。 CD24在低分化腺癌中的表达高于中+高分化腺癌(P<0．05)。 CD24与肿瘤的浸润深度、淋巴结转移情况、临床分期及神经受侵密切相关( P<0．05),与患者年龄、性别,有无瘤栓及残端状况无关( P>0．05)。 CD24在两种病理形态学分型中的表达,Lauren分型：弥漫型高于肠型；WHO分型：管状腺癌、乳头状腺癌、黏液腺癌及低黏附型癌之间存在差别,差异有统计学意义(P<0．05)。(2)RT-PCR检测结果：CD24在贲门腺癌中的表达高于癌旁组织(P<0．05),在癌旁组织中微量表达。低分化组相对表达量高于中+高分化组(P<0．05)。 CD24表达与临床分期相关,腺癌中Ⅰ~Ⅳ期CD24 mRNA的相对表达量逐级升高,差异有统计学意义(P<0．05),结果与免疫表型一致。结论 CD24与肿瘤的分化程度、浸润深度、淋巴结是否转移及临床分期密切相关,提示CD24可能参与贲门腺癌的恶性过程,其表达越高肿瘤分化越差。
목적：검측종류간세포표지물CD24재140례분문선암중적표체,탐토기여선암림상병이학삼수적관계。방법채용면역조화SP법검측140례분문선암중CD24적표체,분석기여림상병이학특정급생물학행위지간적상관성；수집신선암조직여암방조직각26례,의거불동림상분기、불동분화정도진행분조,분별제취량조중적총RNA진행RT-PCR검측,계산CD24적상대표체량,분석량조간적차이성,진일보분석이자적침습성。결과(1)면역조화검측결과：CD24재암조직중적표체고우암방조직(P<0．05)。 CD24재저분화선암중적표체고우중+고분화선암(P<0．05)。 CD24여종류적침윤심도、림파결전이정황、림상분기급신경수침밀절상관( P<0．05),여환자년령、성별,유무류전급잔단상황무관( P>0．05)。 CD24재량충병리형태학분형중적표체,Lauren분형：미만형고우장형；WHO분형：관상선암、유두상선암、점액선암급저점부형암지간존재차별,차이유통계학의의(P<0．05)。(2)RT-PCR검측결과：CD24재분문선암중적표체고우암방조직(P<0．05),재암방조직중미량표체。저분화조상대표체량고우중+고분화조(P<0．05)。 CD24표체여림상분기상관,선암중Ⅰ~Ⅳ기CD24 mRNA적상대표체량축급승고,차이유통계학의의(P<0．05),결과여면역표형일치。결론 CD24여종류적분화정도、침윤심도、림파결시부전이급림상분기밀절상관,제시CD24가능삼여분문선암적악성과정,기표체월고종류분화월차。
Purpose To investigate the expression of CD24 in cardiac adenocarcinoma, and to explore the relationship between the two molecules and other clinicopathological features. Methods The expression of CD24 in 140 cases of cardiac adenocarcinoma was detec-ted by using immunohistochemistry. And the relationship between CD24 and clinicopathological features, adenocarcinoma biological be-havior were analyzed, total RNAs were extracted from 26 cases of fresh carcinoma tissues and adjacent mucosa, each groups were divid-ed by different clinical staging, different degree of differentiation. The expression of CD24 was detected by using RT-PCR and the re-sults were shown as relative quantity. The difference of CD24 expression between two groups was further analyzed and the invasion of both groups was compared. Results Immunohistochemically, the expression of CD24 was significantly higher in carcinoma tissues than that in adjacent mucosa (P<0. 05), and those expression was associated with tumor differentiation (P<0. 05). CD24 was also closely related with the invasion, lymph node metastasis, clinical stages and nerves involvement (P<0. 05), while no relation with age, gender, vascular blots and residual situation (P>0. 05), the expression of CD24 in two pathological types showed that Lauren diffuse type was higher than intestinal type, the expression of CD24 was different among tubular adenocarcinoma, polypoid adenocarci-noma, mucinous adenocarcinoma and low adhesion adenocarcinoma (WHO classification) (P<0. 05). RT-PCR results demonstrated that the expression of CD24 was markedly higher in carcinoma tissues than those in adjacent mucosa ( P<0. 05 ) . The expression of CD24 in poorly differentiated adenocarcinoma was significantly higher than those in intermediate/highly differentiated adenocarcinoma (P<0. 05). CD24 expression was also associated with clinical stages. The relative quantity from stageⅠtoⅣwas progressively ris-ing (P<0. 05). Conclusions The expression of CD24 is closely related with the tumor differentiation, invasion, lymph node metas-tasis and clinical staging, suggesting that CD24 may be involved in the progression of cardiac adenocarcinoma, and the patients with higher CD24 expression may have poorer prognosis.