CAJ | 학술논문

目的：探讨CIK细胞对肿瘤组织的靶向性及其在荷瘤鼠体内的运行轨迹和对主要脏器的影响。方法分离制备人CIK细胞,采用体外Transwell迁移试验观察CIK细胞对人乳腺癌细胞MDA-MB-435的靶向迁移效应；以该细胞系接种BALB/c裸鼠建立乳腺癌移植瘤模型,尾静脉注射DiI标记的CIK细胞,应用活体成像和病理学检查观察CIK细胞运行轨迹及对主要脏器的影响。结果 CIK细胞在体外培养14~20天时,细胞增殖达到高峰,CD3+CD56+ T细胞的比例也达到最高值。体外Tr-answell迁移实验显示,随着肿瘤细胞上清液浓度的增加,CIK细胞的迁移数量也随之增多。荷瘤裸鼠尾静脉注射DiI标记的CIK细胞后,活体成像显示肿瘤组织24 h开始出现荧光信号,48 h达到最强；HE和免疫组化染色结果显示,注射后6 h肿瘤周围开始聚集CIK细胞,48 h最多,第14天时肿瘤部位仍有CIK细胞存在,各脏器均未发现由CIK细胞导致的病理组织学损伤。结论 CIK细胞在体内外对肿瘤组织均具有靶向性,对正常组织有安全性,可作为一种有潜力的细胞载体应用于肿瘤的靶向治疗。
목적：탐토CIK세포대종류조직적파향성급기재하류서체내적운행궤적화대주요장기적영향。방법분리제비인CIK세포,채용체외Transwell천이시험관찰CIK세포대인유선암세포MDA-MB-435적파향천이효응；이해세포계접충BALB/c라서건립유선암이식류모형,미정맥주사DiI표기적CIK세포,응용활체성상화병이학검사관찰CIK세포운행궤적급대주요장기적영향。결과 CIK세포재체외배양14~20천시,세포증식체도고봉,CD3+CD56+ T세포적비례야체도최고치。체외Tr-answell천이실험현시,수착종류세포상청액농도적증가,CIK세포적천이수량야수지증다。하류라서미정맥주사DiI표기적CIK세포후,활체성상현시종류조직24 h개시출현형광신호,48 h체도최강；HE화면역조화염색결과현시,주사후6 h종류주위개시취집CIK세포,48 h최다,제14천시종류부위잉유CIK세포존재,각장기균미발현유CIK세포도치적병리조직학손상。결론 CIK세포재체내외대종류조직균구유파향성,대정상조직유안전성,가작위일충유잠력적세포재체응용우종류적파향치료。
Purpose To evaluate the tumor tropism of cytokine-induced killer ( CIK) cells, the movement track in nude mice bearing breast carcinoma and the influence on major organs of nude mice. Methods Separated and prepared CIK cells using human peripheral blood. The transwell migration assay was used to study the migratory response of CIK cells to human MDA-MB-435 breast carcinoma cells. A nude mouse xenograft model ( BALB/c) was established by injection of human MDA-MB-435 breast carcinoma cells. CIK cells labelled with DiI were injected into caudal vein of the nude mice bearing transplantation tumor. Movement track of CIK cells in vi-vo and influence on major organs were observed by living imaging technology, histopathology and immunohistopathology. Results When cultured in vitro during 14 ~20 days, CIK cells reached the peak level in proliferating stage with the maximum proportion of CD3 +CD56 + T cells. Transwell migration assay showed that the migrating number of CIK cells was increasing along with the increasing concentration of tumor cell cultural supernatants. Living imaging technology showed that the fluorescence signal began to appear 24 hours after injection of CIK cells and was strongest at 48 hours. Immunohistochemical technique and hematoxylin-eosin stain showed CIK cells tended to gather around tumor tissue 6 hours after injection, the most at 48 hours, and with some of the remaining cells on 14 day. In the meantime, no pathological damage caused by CIK cells was observed. Conclusion CIK cells have good tropism to the tumor tissue and safety to the normal tissue, and could be used as a promising cell vector for targeted therapy of cancer.