CAJ | 학술논문

目的：利用各种生物信息学工具预测hsa-miR-1908上游启动子功能，以进一步研究其在人脂肪细胞中的转录调控机制。方法应用Ensemble数据库获取hsa-miR-1908上游启动子序列，利用多种在线相关软件预测出甲基化部位和转录因子结合部位。结果获取hsa-miR-1908上游启动子序列全长1458 bp。miR-1908启动子序列中CpG岛位于（438～756）bp、（836～937）bp、（979～1374）bp处，CpG岛的存在会抑制miR-1908启动子的转录。miR-1908有15个转录因子的结合位点。结论 miRNA启动子相关生物信息学的研究，提高对启动子的研究效率，为进一步研究miR-1908的转录调控机制提供了重要的信息。
목적：이용각충생물신식학공구예측hsa-miR-1908상유계동자공능，이진일보연구기재인지방세포중적전록조공궤제。방법응용Ensemble수거고획취hsa-miR-1908상유계동자서렬，이용다충재선상관연건예측출갑기화부위화전록인자결합부위。결과획취hsa-miR-1908상유계동자서렬전장1458 bp。miR-1908계동자서렬중CpG도위우（438～756）bp、（836～937）bp、（979～1374）bp처，CpG도적존재회억제miR-1908계동자적전록。miR-1908유15개전록인자적결합위점。결론 miRNA계동자상관생물신식학적연구，제고대계동자적연구효솔，위진일보연구miR-1908적전록조공궤제제공료중요적신식。
Objective To predict the functions of hsa-miR-1908 promoter using various bioinformatic tools, and to provide clues for further study on transcriptional regulation mechanism of miR-1908 in human adipocytes. Methods The promoter se-quence of miR-1908 was obtained from Ensemble, and then the CpG islands and transcription factor binding sites were pre-dicted by a variety of online bioinformatic tools. Results The length of the miR-1908 promoter sequence was 1 458 bp. The CpG islands, which inhibited the transcription of miR-1908, were located at (438-756) bp, (836-937) bp and (979-1374) bp. Meanwhile, 15 transcription factor binding sites were found in the promoter sequence of miR-1908. Conclusions miRNA up-stream promoter related bioinformatics can not only improve the efficiency of microRNA promoter research, but also provide further important information on transcriptional regulation of miR-1908.