临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
4期
364-367
,共4页
表皮葡萄球菌%脑组织%新生小鼠
錶皮葡萄毬菌%腦組織%新生小鼠
표피포도구균%뇌조직%신생소서
Staphylococcus epidermidis%brain tissue%newborn mouse
目的：探讨表皮葡萄球菌（Staphylococcus epidermidis，SE）感染对不同日龄新生小鼠的影响。方法出生后第1天（PND1）和第3天（PND3）新生小鼠共60只，分为SE组、生理盐水（NS）组、对照组，每组20只。SE组静脉注射108/ml的SE 50μl，NS组给予等量NS，对照组不作任何处理。第14天时取脑、肝、脾称重，脑组织石蜡包埋后连续切片，用于微管相关蛋白-2（microtubule associated protein-2，MAP-2）和髓鞘碱性蛋白（myelin basic protein，MBP）免疫组化染色，并计算脑灰质和白质面积和体积。结果 PND1小鼠SE组的死亡率为60.0%，NS组为40.0%，差异无统计学意义（P>0.05）；PND3小鼠SE组的死亡率10.0%，NS组无死亡，差异无统计学意义（P>0.05）。PND1和PND3组的体质量，体质量增长，肝、脾重量及脏器系数的差异无统计学意义（P均>0.05）。PND1小鼠SE组的脑灰质面积和体积明显低于NS组，脑白质面积和体积也低于NS组，差异有统计学意义（P均<0.05）；PND3小鼠SE组脑灰质及脑白质的面积和体积与NS组的差异无统计学意义（P均>0.05）。结论 SE感染可致PND1小鼠的脑组织损伤，而对PND3小鼠无明显影响。
目的：探討錶皮葡萄毬菌（Staphylococcus epidermidis，SE）感染對不同日齡新生小鼠的影響。方法齣生後第1天（PND1）和第3天（PND3）新生小鼠共60隻，分為SE組、生理鹽水（NS）組、對照組，每組20隻。SE組靜脈註射108/ml的SE 50μl，NS組給予等量NS，對照組不作任何處理。第14天時取腦、肝、脾稱重，腦組織石蠟包埋後連續切片，用于微管相關蛋白-2（microtubule associated protein-2，MAP-2）和髓鞘堿性蛋白（myelin basic protein，MBP）免疫組化染色，併計算腦灰質和白質麵積和體積。結果 PND1小鼠SE組的死亡率為60.0%，NS組為40.0%，差異無統計學意義（P>0.05）；PND3小鼠SE組的死亡率10.0%，NS組無死亡，差異無統計學意義（P>0.05）。PND1和PND3組的體質量，體質量增長，肝、脾重量及髒器繫數的差異無統計學意義（P均>0.05）。PND1小鼠SE組的腦灰質麵積和體積明顯低于NS組，腦白質麵積和體積也低于NS組，差異有統計學意義（P均<0.05）；PND3小鼠SE組腦灰質及腦白質的麵積和體積與NS組的差異無統計學意義（P均>0.05）。結論 SE感染可緻PND1小鼠的腦組織損傷，而對PND3小鼠無明顯影響。
목적：탐토표피포도구균（Staphylococcus epidermidis，SE）감염대불동일령신생소서적영향。방법출생후제1천（PND1）화제3천（PND3）신생소서공60지，분위SE조、생리염수（NS）조、대조조，매조20지。SE조정맥주사108/ml적SE 50μl，NS조급여등량NS，대조조불작임하처리。제14천시취뇌、간、비칭중，뇌조직석사포매후련속절편，용우미관상관단백-2（microtubule associated protein-2，MAP-2）화수초감성단백（myelin basic protein，MBP）면역조화염색，병계산뇌회질화백질면적화체적。결과 PND1소서SE조적사망솔위60.0%，NS조위40.0%，차이무통계학의의（P>0.05）；PND3소서SE조적사망솔10.0%，NS조무사망，차이무통계학의의（P>0.05）。PND1화PND3조적체질량，체질량증장，간、비중량급장기계수적차이무통계학의의（P균>0.05）。PND1소서SE조적뇌회질면적화체적명현저우NS조，뇌백질면적화체적야저우NS조，차이유통계학의의（P균<0.05）；PND3소서SE조뇌회질급뇌백질적면적화체적여NS조적차이무통계학의의（P균>0.05）。결론 SE감염가치PND1소서적뇌조직손상，이대PND3소서무명현영향。
Objectives To study the influence of Staphylococcus epidermidis (SE) on the neonatal mice of different ages. Methods A total of 60 neonatal mice including postnatal day 1(PND1) and postnatal day 3(PND3) were divided into SE group, normal saline (NS) group and control group, with 20 mice each. Mice in SE group were intravenously injected with 50μl SE (108/ml). Mice in NS group were given 50μl NS and mice in control group was not intervened. On postnatal day 14, the brain, liver and spleen obtained from mice were weighted. Serial sections of paraffin-embedded brain tissue were used for the detec-tion of microtubule associated protein-2 (MAP-2) and myelin basic protein (MBP) by immumohistochemical staining, and then the areas and volumes of grey and white matter were calculated. Result The mortality of PND1 mice in SE and NS group was 60.0%and 40.0%, respectively, and there was no difference between two groups (P>0.05). The mortality of PND3 mice in SE and NS group was 10.0%and 0.0%, respectively, and there was no difference between two groups (P>0.05). There were no dif-ferences in body weight, body weight gain, spleen and liver weights and organ coefficient between PND1 and PND3 mice (P>0.05). In PND1 mice, the areas and volumes of grey and white matter were significantly smaller in SE group than those in NS group (P<0.05). However, in PND3 mice, there was no differences in areas and volumes of grey and white matter between SE and NS group (P>0.05). Conclusions SE infection can result in brain injury in PND1 mice, but has no effect on brain tissues of PND3 mice.
