临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
4期
360-363
,共4页
白介素-23%白介素-17%病毒性心肌炎%小鼠
白介素-23%白介素-17%病毒性心肌炎%小鼠
백개소-23%백개소-17%병독성심기염%소서
interleukin-23%interleukin-17%viral myocarditis%mouse
目的：观察白介素（IL）-23/IL-17炎症轴在病毒性心肌炎（VMC）小鼠中表达的动态变化，并探讨其意义。方法将60只BALB/c小鼠随机分为VMC组（n=50）和对照组（n=10）。VMC组腹腔注射0.1 ml含柯萨奇B3病毒（CVB3）的Eagle’s液建立VMC模型，分别于接种后第7、14、28天随机各取10只处死；对照组腹腔注射0.1 ml不含CVB3的Eagle’s液，于第28天处死。通过苏木精-伊红（HE）染色检查心肌病理改变，并计算心肌病理积分；采用荧光实时定量PCR、Western blotting检测心肌IL-23及IL-17的mRNA、蛋白表达水平；采用酶联免疫吸附法测定血清IL-23、IL-17浓度；并对VMC组血清IL-23、IL-17浓度与心肌病变积分之间的相关性进行分析。结果 VMC组接种病毒后第7、14、28天心肌病理积分， IL-23和IL-17 mRNA及蛋白表达水平，以及血清IL-23、IL-17水平均高于对照组，差异有统计学意义（P均<0.01）；其中接种病毒后第7天，VCM组的各项指标均处于最高水平，随后逐渐下降，在各时间点各项指标的差异均有统计学意义（P均<0.05）；VMC组的血清IL-23、IL-17水平与心肌病理积分呈正相关（r=0.73、0.76，P均<0.05）。结论 IL-23/IL-17炎症轴在VMC的发病过程中可能起重要作用，并与VMC病情严重程度密切相关。
目的：觀察白介素（IL）-23/IL-17炎癥軸在病毒性心肌炎（VMC）小鼠中錶達的動態變化，併探討其意義。方法將60隻BALB/c小鼠隨機分為VMC組（n=50）和對照組（n=10）。VMC組腹腔註射0.1 ml含柯薩奇B3病毒（CVB3）的Eagle’s液建立VMC模型，分彆于接種後第7、14、28天隨機各取10隻處死；對照組腹腔註射0.1 ml不含CVB3的Eagle’s液，于第28天處死。通過囌木精-伊紅（HE）染色檢查心肌病理改變，併計算心肌病理積分；採用熒光實時定量PCR、Western blotting檢測心肌IL-23及IL-17的mRNA、蛋白錶達水平；採用酶聯免疫吸附法測定血清IL-23、IL-17濃度；併對VMC組血清IL-23、IL-17濃度與心肌病變積分之間的相關性進行分析。結果 VMC組接種病毒後第7、14、28天心肌病理積分， IL-23和IL-17 mRNA及蛋白錶達水平，以及血清IL-23、IL-17水平均高于對照組，差異有統計學意義（P均<0.01）；其中接種病毒後第7天，VCM組的各項指標均處于最高水平，隨後逐漸下降，在各時間點各項指標的差異均有統計學意義（P均<0.05）；VMC組的血清IL-23、IL-17水平與心肌病理積分呈正相關（r=0.73、0.76，P均<0.05）。結論 IL-23/IL-17炎癥軸在VMC的髮病過程中可能起重要作用，併與VMC病情嚴重程度密切相關。
목적：관찰백개소（IL）-23/IL-17염증축재병독성심기염（VMC）소서중표체적동태변화，병탐토기의의。방법장60지BALB/c소서수궤분위VMC조（n=50）화대조조（n=10）。VMC조복강주사0.1 ml함가살기B3병독（CVB3）적Eagle’s액건립VMC모형，분별우접충후제7、14、28천수궤각취10지처사；대조조복강주사0.1 ml불함CVB3적Eagle’s액，우제28천처사。통과소목정-이홍（HE）염색검사심기병리개변，병계산심기병리적분；채용형광실시정량PCR、Western blotting검측심기IL-23급IL-17적mRNA、단백표체수평；채용매련면역흡부법측정혈청IL-23、IL-17농도；병대VMC조혈청IL-23、IL-17농도여심기병변적분지간적상관성진행분석。결과 VMC조접충병독후제7、14、28천심기병리적분， IL-23화IL-17 mRNA급단백표체수평，이급혈청IL-23、IL-17수평균고우대조조，차이유통계학의의（P균<0.01）；기중접충병독후제7천，VCM조적각항지표균처우최고수평，수후축점하강，재각시간점각항지표적차이균유통계학의의（P균<0.05）；VMC조적혈청IL-23、IL-17수평여심기병리적분정정상관（r=0.73、0.76，P균<0.05）。결론 IL-23/IL-17염증축재VMC적발병과정중가능기중요작용，병여VMC병정엄중정도밀절상관。
Objective To observe the dynamic changes of interleukin-23 (IL-23)/interleukin-17 (IL-17) inflammatory axis expression in the myocardium of mice with viral myocarditis (VM) and further to explore its significance. Methods A total of 60 BALB/c mice were randomly divided into VMC group (n=50) and control group (n=10). Mice in VMC group were intraperito-neally inoculated with 0.1ml Eagle's solution containing coxsackievirus B3 (CVB3) to establish VM model and 10 mice were sacri-ficed each time at days 7, 14 and 28 after inoculation. Mice in control group were treated with 0.1ml Eagle's solution without CVB3 and all the 30 mice were killed on day 28 after inoculation. Myocardial histopathologic changes were detected with hema-toxylin and eosin (HE) staining and myocardial histopathological scores were calculated. The expression levels of myocardial IL-23/IL-17 mRNA and protein were determined by real-time quantitative PCR and immunohistochemistry, respectively. Serum IL-23 and IL-17 concentrations were examined using ELISA. Also, the relationship between serum IL-23 and IL-17 concentra-tions and myocardial histopathological scores was analyzed in VMC group. Results At days 7, 14 and 28 after inoculation, the myocardial histopathological scores, mRNA and protein expressions of myocardial IL-23/IL-17 and serum IL-23 and IL-17 con-centrations were significantly higher in VMC group than those in control group (P<0.01). Each of the above indicators reached its highest level on 7th day after inoculation (P<0.01) and then gradually decreased. There were significant differences among dif-ferent time points (P<0.05). Serum IL-23 and IL-17 concentrations showed significantly positive correlation with the myocardi-al histopathologic scores in VMC group, respectively (r=0.73 and 0.76, P<0.05). Conclusions IL-23/IL-17 inflammatory axis may play an important role in the process of VMC and is closely associated with the severity of VMC.
