临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
4期
355-359
,共5页
支气管肺发育不良%高氧%血管生成素
支氣管肺髮育不良%高氧%血管生成素
지기관폐발육불량%고양%혈관생성소
bronchopulmonary dysplasia%hyperoxia%angiopoietin
目的：探讨血管生成素（Ang-1）在高氧诱导支气管肺发育不良（BDP）新生大鼠中的表达。方法48只生后2~3日龄的新生大鼠随机分为高氧组和对照组，每组24只，分别置于高氧（氧浓度≥95%）和空气中持续喂养；于高氧暴露第1、3、7天，光镜下观察大鼠肺组织形态结构变化，并采用实时荧光定量PCR和Western blotting方法分别检测大鼠肺组织中Ang-1 mRNA和蛋白表达水平。结果随着高氧暴露时间延长，光镜下高氧组逐渐表现出肺组织发育不良、肺泡结构简单化、肺泡数目减少和肺微血管发育受阻等病理改变。高氧暴露第7天，高氧组的Ang-1 mRNA和蛋白相对表达为0.33±0.18和0.20±0.07，低于对照组的0.83±0.46和0.57±0.44，差异有统计学意义（P均<0.05）。结论 Ang-1是肺血管发育过程中的重要调节因子，参与了BPD病理发生。
目的：探討血管生成素（Ang-1）在高氧誘導支氣管肺髮育不良（BDP）新生大鼠中的錶達。方法48隻生後2~3日齡的新生大鼠隨機分為高氧組和對照組，每組24隻，分彆置于高氧（氧濃度≥95%）和空氣中持續餵養；于高氧暴露第1、3、7天，光鏡下觀察大鼠肺組織形態結構變化，併採用實時熒光定量PCR和Western blotting方法分彆檢測大鼠肺組織中Ang-1 mRNA和蛋白錶達水平。結果隨著高氧暴露時間延長，光鏡下高氧組逐漸錶現齣肺組織髮育不良、肺泡結構簡單化、肺泡數目減少和肺微血管髮育受阻等病理改變。高氧暴露第7天，高氧組的Ang-1 mRNA和蛋白相對錶達為0.33±0.18和0.20±0.07，低于對照組的0.83±0.46和0.57±0.44，差異有統計學意義（P均<0.05）。結論 Ang-1是肺血管髮育過程中的重要調節因子，參與瞭BPD病理髮生。
목적：탐토혈관생성소（Ang-1）재고양유도지기관폐발육불량（BDP）신생대서중적표체。방법48지생후2~3일령적신생대서수궤분위고양조화대조조，매조24지，분별치우고양（양농도≥95%）화공기중지속위양；우고양폭로제1、3、7천，광경하관찰대서폐조직형태결구변화，병채용실시형광정량PCR화Western blotting방법분별검측대서폐조직중Ang-1 mRNA화단백표체수평。결과수착고양폭로시간연장，광경하고양조축점표현출폐조직발육불량、폐포결구간단화、폐포수목감소화폐미혈관발육수조등병리개변。고양폭로제7천，고양조적Ang-1 mRNA화단백상대표체위0.33±0.18화0.20±0.07，저우대조조적0.83±0.46화0.57±0.44，차이유통계학의의（P균<0.05）。결론 Ang-1시폐혈관발육과정중적중요조절인자，삼여료BPD병리발생。
Objective To explore the expression of angiopoietin-1 (Ang-1) and lung development in neonatal rat with hy-peroxia-induced bronchopulmonary dysplasia (BPD). Methods A total of forty-eight 1-to 3-day-old neonatal rats were random-ly divided into hyperoxia group and control group with 24 rats in each group, fed in high concentration oxygen (≥95%) or in air respectively. At 1st, 3rd and 7th day after high oxygen exposure, the histological changes in lung tissue were observed by HE stai-ning under a light microscope and the expressions of Ang-1 mRNA and its protein in lung tissue were detected by RT-PCR and Western blot. Results With extended exposure to high concentrations of oxygen, rats in hyperoxia group presented such patho-logic change of lung tissue dysplasia as alveolar simplification, reduction in alveolar number and arrested pulmonary microvas-cular development. At 7th day after high oxygen exposure, Ang-1 mRNA and protein expressions in hyperoxia group were (0.33± 0.18) and (0.20±0.07), significantly lower than those [(0.83±0.46) and (0.57±0.44)] in control group (P<0.05). Conclusions Ang-1 plays an important regulatory role in the pulmonary vascular development and participates in the pathogenesis of BPD.
