CAJ | 학술논문

本试验旨在探讨金雀异黄素( genistein,GEN)对多囊卵巢综合征( polycystic ovary syn-drome,PCOS)大鼠卵巢组织中抗凋亡基因Bcl-2及促凋亡基因Bax mRNA表达的影响。将90只雌性Wistar大鼠随机分为6组：对照组,模型组,GEN低、中、高剂量组及雌激素组。按照胰岛素联合人体绒毛膜促性腺激素造模法建立PCOS试验动物模型,造模成功后,每组选择10只大鼠进行试验。剂量组分别给予5、10、20 mg/kg GEN,雌激素组给予0.5 mg/kg己烯雌酚,受试物灌胃给予,持续15 d,观察动情周期10 d。采用原位杂交法检测各组大鼠卵巢组织中Bcl-2和Bax mRNA表达量。同时测定大鼠卵巢组织中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性及丙二醛含量。结果表明：与模型组相比, GEN能提高大鼠卵巢组织中Bcl-2 mRNA表达量,降低Bax mRNA表达量,并且可调节卵巢组织中抗氧化酶活性,其作用效果与雌激素相似。 GEN可以增强大鼠卵巢组织中 Bcl-2 mRNA 表达量,拮抗 Bax mRNA 表达量,推测这是GEN改善PCOS卵巢功能的作用途径之一。
본시험지재탐토금작이황소( genistein,GEN)대다낭란소종합정( polycystic ovary syn-drome,PCOS)대서란소조직중항조망기인Bcl-2급촉조망기인Bax mRNA표체적영향。장90지자성Wistar대서수궤분위6조：대조조,모형조,GEN저、중、고제량조급자격소조。안조이도소연합인체융모막촉성선격소조모법건립PCOS시험동물모형,조모성공후,매조선택10지대서진행시험。제량조분별급여5、10、20 mg/kg GEN,자격소조급여0.5 mg/kg기희자분,수시물관위급여,지속15 d,관찰동정주기10 d。채용원위잡교법검측각조대서란소조직중Bcl-2화Bax mRNA표체량。동시측정대서란소조직중초양화물기화매、과양화경매、곡광감태과양화물매활성급병이철함량。결과표명：여모형조상비, GEN능제고대서란소조직중Bcl-2 mRNA표체량,강저Bax mRNA표체량,병차가조절란소조직중항양화매활성,기작용효과여자격소상사。 GEN가이증강대서란소조직중 Bcl-2 mRNA 표체량,길항 Bax mRNA 표체량,추측저시GEN개선PCOS란소공능적작용도경지일。
This experiment was conducted to study the effects of genistein ( GEN) on the expressions of anti-apoptotic gene Bcl-2 mRNA and pro-apoptotic gene Bax mRNA in ovarian tissue of polycystic ovary syndrome ( PCOS) rats. Ninety rats were divided into six groups: control group, model group, GEN low dose group, GEN middle dose group, GEN high dose group and estrogen group, respectively. The insulin combined with human chorionic gonadotropin molding method was used to establish PCOS animal models. After modeling, ten rats were selected from each group to carry out the test. The rats in dose groups were given 5 , 10 and 20 mg/kg GEN, respectively, and the rats in estrogen group were given 0. 5 mg/kg diethylstilbestrol. All compounds were administered intragastric administration on 15 consecutive days and observed a estrous cycle of 10 days. The expressions of Bcl-2 and Bax mRNA in rat ovarian tissue were examined by in situ hybridization. The activities of superoxide dismutase, catalase and glutathione peroxidase, and malondialdehyde content in rat ovarian tissue were determined. The results showed as follows: compared with PCOS model group, GEN could raise the expression of Bcl-2 mRNA and weaken the expression of Bax mRNA. And GEN could regulate the activities of antioxidant enzymes in rat ovarian tissue, which was as the same as the estrogen. In conclu-sion, GEN can enhance the expression of Bcl-2 mRNA and reduce the expression of Bax mRNA in rat ovarian tissue. It is presumed that it is one of the mechanisms of GEN improving ovarian function of PCOS.