中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2013年
7期
513-518
,共6页
王玉阳%王佩佩%张振燕%王强
王玉暘%王珮珮%張振燕%王彊
왕옥양%왕패패%장진연%왕강
强化训练%脑缺血%再灌注%蛋白激酶类%神经再生
彊化訓練%腦缺血%再灌註%蛋白激酶類%神經再生
강화훈련%뇌결혈%재관주%단백격매류%신경재생
Intensive training%Cerebral ischemia%Reperfusion%Protein kinases%Nerve regeneration
目的 探讨强化游泳训练对脑缺血再灌注大鼠神经功能及蛋白激酶A(PKA)表达的影响.方法 采用线栓法建立左侧大脑中动脉阻塞(MCAO)2 h再灌注动物模型.120只造模成功的雄性SD大鼠按随机数字表法分为训练1组、训练2组、训练3组和MCAO对照组,每组30只.训练1组大鼠每日游泳5 min,训练2组大鼠每日游泳10 min,训练3组大鼠每日游泳20 min;MCAO对照组大鼠不给予任何训练.另选30只大鼠为假手术组,不阻塞大脑中动脉血流,不给予任何训练.以上5组大鼠又分别随机分为术后3、7和14 d三个时间点亚组,每个时间点10只大鼠.分别于术后3、7和14d三个时间点,采用Menzies神经行为评分和前肢放置试验评价各亚组大鼠神经功能缺损情况,采用免疫组织化学方法测定各亚组大鼠脑缺血再灌注后脑内PKA阳性细胞的表达率,采用RT-PCR的方法测定各亚组大鼠脑缺血侧大脑皮质PKA的mRNA表达量,并进行统计学分析比较.结果 假手术组神经功能正常.MCAO对照组各时间点的神经功能评分与假手术组同时间点比较,组间差异均有统计学意义(P＜0.05);各训练组分别在训练3、7和14 d后的神经功能改善情况优于同时间点MCAO对照组;训练3组训练14 d后受损神经功能改善最明显(P＜0.05),其训练3、7和14 d后的Menzies评分分别为(1.40±0.55)、(1.00±0.71)和(0.80±0.45)分,前肢放置试验评分分别为(5.20±0.84)、(3.80±1.30)和(2.40±1.14)分.训练1、2和3组各时间点的PKA阳性细胞表达率及其mRNA表达量高于同时间点MCAO对照组;且训练3组训练14d后的PKA阳性细胞表达率及其mRNA表达量增加较其它各训练组更为明显(P＜0.05),其训练3、7和14 d后的PKA阳性细胞表达率分别为(32.84±1.66)％、(35.05±1.33)％和(45.92 ±2.03)％,PKA mRNA表达量分别为(32.18±0.86)％、(37.44±1.27)％和(42.77±0.81)％.结论 强化游泳训练可以增加脑缺血再灌注大鼠PKA阳性细胞表达率及其mRNA表达量,改善受损神经功能,促进感觉和运动功能恢复.
目的 探討彊化遊泳訓練對腦缺血再灌註大鼠神經功能及蛋白激酶A(PKA)錶達的影響.方法 採用線栓法建立左側大腦中動脈阻塞(MCAO)2 h再灌註動物模型.120隻造模成功的雄性SD大鼠按隨機數字錶法分為訓練1組、訓練2組、訓練3組和MCAO對照組,每組30隻.訓練1組大鼠每日遊泳5 min,訓練2組大鼠每日遊泳10 min,訓練3組大鼠每日遊泳20 min;MCAO對照組大鼠不給予任何訓練.另選30隻大鼠為假手術組,不阻塞大腦中動脈血流,不給予任何訓練.以上5組大鼠又分彆隨機分為術後3、7和14 d三箇時間點亞組,每箇時間點10隻大鼠.分彆于術後3、7和14d三箇時間點,採用Menzies神經行為評分和前肢放置試驗評價各亞組大鼠神經功能缺損情況,採用免疫組織化學方法測定各亞組大鼠腦缺血再灌註後腦內PKA暘性細胞的錶達率,採用RT-PCR的方法測定各亞組大鼠腦缺血側大腦皮質PKA的mRNA錶達量,併進行統計學分析比較.結果 假手術組神經功能正常.MCAO對照組各時間點的神經功能評分與假手術組同時間點比較,組間差異均有統計學意義(P＜0.05);各訓練組分彆在訓練3、7和14 d後的神經功能改善情況優于同時間點MCAO對照組;訓練3組訓練14 d後受損神經功能改善最明顯(P＜0.05),其訓練3、7和14 d後的Menzies評分分彆為(1.40±0.55)、(1.00±0.71)和(0.80±0.45)分,前肢放置試驗評分分彆為(5.20±0.84)、(3.80±1.30)和(2.40±1.14)分.訓練1、2和3組各時間點的PKA暘性細胞錶達率及其mRNA錶達量高于同時間點MCAO對照組;且訓練3組訓練14d後的PKA暘性細胞錶達率及其mRNA錶達量增加較其它各訓練組更為明顯(P＜0.05),其訓練3、7和14 d後的PKA暘性細胞錶達率分彆為(32.84±1.66)％、(35.05±1.33)％和(45.92 ±2.03)％,PKA mRNA錶達量分彆為(32.18±0.86)％、(37.44±1.27)％和(42.77±0.81)％.結論 彊化遊泳訓練可以增加腦缺血再灌註大鼠PKA暘性細胞錶達率及其mRNA錶達量,改善受損神經功能,促進感覺和運動功能恢複.
목적 탐토강화유영훈련대뇌결혈재관주대서신경공능급단백격매A(PKA)표체적영향.방법 채용선전법건립좌측대뇌중동맥조새(MCAO)2 h재관주동물모형.120지조모성공적웅성SD대서안수궤수자표법분위훈련1조、훈련2조、훈련3조화MCAO대조조,매조30지.훈련1조대서매일유영5 min,훈련2조대서매일유영10 min,훈련3조대서매일유영20 min;MCAO대조조대서불급여임하훈련.령선30지대서위가수술조,불조새대뇌중동맥혈류,불급여임하훈련.이상5조대서우분별수궤분위술후3、7화14 d삼개시간점아조,매개시간점10지대서.분별우술후3、7화14d삼개시간점,채용Menzies신경행위평분화전지방치시험평개각아조대서신경공능결손정황,채용면역조직화학방법측정각아조대서뇌결혈재관주후뇌내PKA양성세포적표체솔,채용RT-PCR적방법측정각아조대서뇌결혈측대뇌피질PKA적mRNA표체량,병진행통계학분석비교.결과 가수술조신경공능정상.MCAO대조조각시간점적신경공능평분여가수술조동시간점비교,조간차이균유통계학의의(P＜0.05);각훈련조분별재훈련3、7화14 d후적신경공능개선정황우우동시간점MCAO대조조;훈련3조훈련14 d후수손신경공능개선최명현(P＜0.05),기훈련3、7화14 d후적Menzies평분분별위(1.40±0.55)、(1.00±0.71)화(0.80±0.45)분,전지방치시험평분분별위(5.20±0.84)、(3.80±1.30)화(2.40±1.14)분.훈련1、2화3조각시간점적PKA양성세포표체솔급기mRNA표체량고우동시간점MCAO대조조;차훈련3조훈련14d후적PKA양성세포표체솔급기mRNA표체량증가교기타각훈련조경위명현(P＜0.05),기훈련3、7화14 d후적PKA양성세포표체솔분별위(32.84±1.66)％、(35.05±1.33)％화(45.92 ±2.03)％,PKA mRNA표체량분별위(32.18±0.86)％、(37.44±1.27)％화(42.77±0.81)％.결론 강화유영훈련가이증가뇌결혈재관주대서PKA양성세포표체솔급기mRNA표체량,개선수손신경공능,촉진감각화운동공능회복.
Objective To observe the effects of intensive-swimming training at different intensities on the neurological function and the expressions of protein kinase A after cerebral ischemia-reperfusion in rats,and to explore the possible neuroprotection mechanism of intensive-swimming training after cerebral ischemia-reperfusion in rats.Methods The animal model of cerebral ischemia-reperfusion was established in rats by using the intraluminal thread method to cause left middle cerebral artery occlusion (MCAO) for 2 h and then reperfusion.A total of 120 male model Sprague-Dawley rats were randomly divided into training group 1 (swimming for 5 min once a day),training group 2 (swimming for 10 min once a day),training group 3 (swimming for 20 min once a day) and control group (no training),with 30 rats in each group.Another 30 rats who were assigned to the sham-operation group were subject to no MCAO and no training.At the 3rd,7th and 14th day post operation,10 rats randomly selected from each group were examined for their neurological function by Menzies neurobehavioral test and forelimb placing test.Expressions of protein kinase A were detected by immunohistochemistry method.Expressions of PKA-mRNA were detected by using RT-PCR.Results The neurological function in sham-operation group was normal.The differences between sham-operation group and control group with regard to Menzies test and forelimb placing test scores were statistically significant at all the time points (P ＜ 0.05).The improvement of neurological function in all training groups,were significantly better than those in controls at the 3rd,7th and 14th d after swimming training,especially in training group 3 on the 14th day post-operation (P ＜0.05).At3rd,7th,and 14th days post-operation,the Menziesscores were(1.40±0.55),(1.00 ±0.71)and (0.80 ±0.45),respectively,and the forelimb placing scores were(5.20 ± 0.84),(3.80 ± 1.30) and (2.40 ± 1.14),respectively,in the training group 3.The number of positive cells and the expressions of protein kinase A mRNA were significantly higher in all training groups than those in the controls at the 3rd,7th and 14th days after swimming training,and the increase of positive cells and mRNA of PKA in the training group 3 was significantly more obvious at 14th day compared with other training groups(P ＜ 0.05).Conclusions Exercise training can increase the expression of positive cells and mRNA of PKA in rats after cerebral ischemiareperfusion,hence,it improved the injured neurological function and promote the recover of sensory and motor function,and the more intensive the training,the better the effects.
