CAJ | 학술논문

本试验旨在研究转基因豆粕对崂山奶山羊生长性能、肌肉营养成分及组织器官中外源基因转移的影响。选取30只50日龄崂山奶山羊随机分为2组，每组3个重复，每个重复5只。精饲料分别以非转基因豆粕和转基因豆粕( MON89788和GTS40-3-2)配制，粗饲料均为干草。试验期150 d。结果表明：2组奶山羊平均日采食量、平均日增重和料重比均无显著差异( P >0.05)；奶山羊肌肉的干物质、粗脂肪、粗蛋白质、粗灰分、钙、磷含量2组间亦均无显著差异( P>0.05)；采用实时定量PCR法检测奶山羊肌肉、肝脏、胰腺、肾脏、脾脏、胸腺、肺、心脏、皱胃、小肠组织的外源基因，均未检测出转基因片段。可见，转基因豆粕( MON89788和GTS40-3-2)对崂山奶山羊生长性能和肌肉品质均无显著影响，转基因豆粕的外源基因在奶山羊肌肉及各组织器官未发生水平转移。
본시험지재연구전기인두박대로산내산양생장성능、기육영양성분급조직기관중외원기인전이적영향。선취30지50일령로산내산양수궤분위2조，매조3개중복，매개중복5지。정사료분별이비전기인두박화전기인두박( MON89788화GTS40-3-2)배제，조사료균위간초。시험기150 d。결과표명：2조내산양평균일채식량、평균일증중화료중비균무현저차이( P >0.05)；내산양기육적간물질、조지방、조단백질、조회분、개、린함량2조간역균무현저차이( P>0.05)；채용실시정량PCR법검측내산양기육、간장、이선、신장、비장、흉선、폐、심장、추위、소장조직적외원기인，균미검측출전기인편단。가견，전기인두박( MON89788화GTS40-3-2)대로산내산양생장성능화기육품질균무현저영향，전기인두박적외원기인재내산양기육급각조직기관미발생수평전이。
In order to analyze the effects of genetically modified soybean meal on growth performance, meat nutrient composition, and exogenous gene transformation to tissues and organs of Laoshan dairy goats, thirty 50-day-old Laoshan dairy goats were randomly divided into two groups with three replicates in each group and five goats per replicate. Goats in different groups were fed concentrates containing non-genetically modified soybean meal and genetically modified soybean meal ( MON89788 and GTS40-3-2 ) , respectively, and were fed the same roughage ( Chinese wildrye) . The experiment lasted for 150 days. The results showed as fol-lows: average daily feed intake, average daily gain and feed to gain ratio in the two groups were not signifi-cantly different (P>0. 05); the contents of dry matter, ether extract, crude protein, ash, calcium and phos-phorus in muscle of dairy goats were not significantly different either ( P >0 . 05 ) . Moreover, no gene rag-ments of genetically modified soybean meal were detected from muscle, liver, pancreas, kidney, spleen, thy-mus, lung, heart, abomasum and small intestine of dairy goats by real-time quantification PCR. The results suggest that genetically modified soybean meal ( MON89788 and GTS40-3-2 ) had no significant effects on growth performance and muscle quality of Laoshan dairy goats, and no genetic horizontal transformation is ob-served in tissues and organs.