动物营养学报
動物營養學報
동물영양학보
ACTA ZOONUTRIMENTA SINICA
2014年
4期
940-946
,共7页
庞德公%杨红建%曹斌斌%武甜甜
龐德公%楊紅建%曹斌斌%武甜甜
방덕공%양홍건%조빈빈%무첨첨
产朊假丝酵母%体外瘤胃发酵%产气动力学参数%全混合日粮
產朊假絲酵母%體外瘤胃髮酵%產氣動力學參數%全混閤日糧
산원가사효모%체외류위발효%산기동역학삼수%전혼합일량
Candida utilis%in vitro rumen fermentation%kinetic parameters of gas production%total mixed ra-tion
本试验将体外瘤胃发酵试验与动态产气实时记录技术相结合,分析了高精料全混合日粮中产朊假丝酵母( Candida utilis,CU)添加水平对体外瘤胃发酵特性和纤维降解的影响。试验用瘤胃液采自5头泌乳期荷斯坦奶牛。试验分为6组,各组CU添加水平分别为0(对照组)、0.53×106、1.07×106、1.60×106、2.13×106、2.67×106 CFU/mL。测定72 h累计产气量、产气动力学参数和瘤胃发酵特性指标。结果表明,随CU添加水平的升高,底物的营养物质(干物质、中性洗涤纤维、酸性洗涤纤维)体外消失率、各时间点产气量、72 h累积产气量、产气动力学参数(理论最大产气量和达到1/2理论最大产气量的时间)、微生物蛋白质与总挥发性脂肪酸浓度以及丙酸、戊酸与支链脂肪酸含量均显著或极显著升高(P<0.050或P<0.001),而乙酸、丁酸含量以及甲烷生成量则极显著降低( P<0.001)。因此,添加CU可提高瘤胃微生物纤维降解与发酵效率。此全混合日粮条件下,CU最适添加水平为2.67×106 CFU/mL。
本試驗將體外瘤胃髮酵試驗與動態產氣實時記錄技術相結閤,分析瞭高精料全混閤日糧中產朊假絲酵母( Candida utilis,CU)添加水平對體外瘤胃髮酵特性和纖維降解的影響。試驗用瘤胃液採自5頭泌乳期荷斯坦奶牛。試驗分為6組,各組CU添加水平分彆為0(對照組)、0.53×106、1.07×106、1.60×106、2.13×106、2.67×106 CFU/mL。測定72 h纍計產氣量、產氣動力學參數和瘤胃髮酵特性指標。結果錶明,隨CU添加水平的升高,底物的營養物質(榦物質、中性洗滌纖維、痠性洗滌纖維)體外消失率、各時間點產氣量、72 h纍積產氣量、產氣動力學參數(理論最大產氣量和達到1/2理論最大產氣量的時間)、微生物蛋白質與總揮髮性脂肪痠濃度以及丙痠、戊痠與支鏈脂肪痠含量均顯著或極顯著升高(P<0.050或P<0.001),而乙痠、丁痠含量以及甲烷生成量則極顯著降低( P<0.001)。因此,添加CU可提高瘤胃微生物纖維降解與髮酵效率。此全混閤日糧條件下,CU最適添加水平為2.67×106 CFU/mL。
본시험장체외류위발효시험여동태산기실시기록기술상결합,분석료고정료전혼합일량중산원가사효모( Candida utilis,CU)첨가수평대체외류위발효특성화섬유강해적영향。시험용류위액채자5두비유기하사탄내우。시험분위6조,각조CU첨가수평분별위0(대조조)、0.53×106、1.07×106、1.60×106、2.13×106、2.67×106 CFU/mL。측정72 h루계산기량、산기동역학삼수화류위발효특성지표。결과표명,수CU첨가수평적승고,저물적영양물질(간물질、중성세조섬유、산성세조섬유)체외소실솔、각시간점산기량、72 h루적산기량、산기동역학삼수(이론최대산기량화체도1/2이론최대산기량적시간)、미생물단백질여총휘발성지방산농도이급병산、무산여지련지방산함량균현저혹겁현저승고(P<0.050혹P<0.001),이을산、정산함량이급갑완생성량칙겁현저강저( P<0.001)。인차,첨가CU가제고류위미생물섬유강해여발효효솔。차전혼합일량조건하,CU최괄첨가수평위2.67×106 CFU/mL。
In vitro ruminal fermentation test and real-time gas production recording technique were combined to determine effects of Candida utilis ( CU) supplemental level in high concentrate total mixed ration on in vitro ruminal fermentation characteristics and fiber degradation. Ruminal fluid was collected from five lactating Hol-stein cows. The CU was supplemented at levels of 0 (control group), 0. 53í106, 1. 07í106, 1. 60 í106, 2. 13í106 and 2. 67 í106 CFU/mL, respectively. The indices of 72 h accumulative gas production, kinetic parameters of gas production and ruminal fermentation characteristics were determined. The results showed as follows: with the increase of CU supplemental level, in vitro disappearance rates of nutrients ( dry matter, neu-tral detergent fiber and acid detergent fiber) in the substrate, gas production at different time points, 72 h accu-mulative gas production, kinetic parameters of gas production ( theoretical maximum gas production and time of reaching 1/2 of it) and microbial protein and total volatile fatty acids concentrations as well as propionic acid, valeric acid and branch-chain fatty acids contents were significantly increased ( P < 0. 050 or P <0 . 001 ) , whereas acetic acid and butyric acid contents and methane production were significantly decreased (P<0. 001). In summary, the supplementation of CU can increase degradation and fermentation of fiber by ruminal microbes. Under conditions of the present total mixed ration, the optimal supplemental level of CU is 2. 67 í 106 CFU/mL.
