CAJ | 학술논문

为了改善酶的刚性固定化对酶蛋白构象的负面影响，以原子转移自由基聚合法(ATRP)合成了亲水性、柔性、触角状环氧化酶固定化载体PS-acyl-P(AM-co-GMA)，通过改变单体总量与引发剂量比例得到不同链长触角状环氧化酶固定化载体，并将其用于耐有机溶剂脂肪酶(YCJ01)的共价柔性固定化，重点考察了链长对固定化酶酶活的影响。结果表明，链长(增重率不超过3200%)越长，酶活越高。
위료개선매적강성고정화대매단백구상적부면영향，이원자전이자유기취합법(ATRP)합성료친수성、유성、촉각상배양화매고정화재체PS-acyl-P(AM-co-GMA)，통과개변단체총량여인발제량비례득도불동련장촉각상배양화매고정화재체，병장기용우내유궤용제지방매(YCJ01)적공개유성고정화，중점고찰료련장대고정화매매활적영향。결과표명，련장(증중솔불초과3200%)월장，매활월고。
In order to improve the enzyme conformation for the negative effect of the rigid immobilized enzyme, the hydrophilic and flexible support PS-acyl-P(acrylamide-co-glycidyl methacrylate) with tentacles-like chain containing epoxy group was prepared with the atom transfer radical polymerization (ATRP) method. A series of chain length of tentacles-like support with epoxy group were preparedvia changing the proportion of the total monomers to the initiator, and were used for flexible immobilization of organic solvent-tolerant lipase (YCJ01) covalently. The major objective of this paper was to investigate the effect of different lengthes of chain on the activity of immobilized lipase. The longer the chain length (weight gain <3200%) was , the higher the activity of immobilized lipase was.