CAJ | 학술논문

为研究玉米秸秆产沼气及其发酵过程中微生物群落变化，以预处理后的玉米秸秆为原料，采用10 L厌氧反应器进行批式中温发酵产沼气。同时取样利用454焦磷酸测序法测定发酵过程中微生物群落的变化。结果表明：该系统启动迅速，在第3 d就达到产气高峰7.78 L，料容产气率为0.97 L·L-1·d-1，46 d原料沼气产率和甲烷产率分别为236.84 ml·(g VS)-1和132.23 ml·(g VS)-1。454焦磷酸测序及分析表明系统中古菌主要为甲烷微菌纲（Methanomicrobia，占总OTU的89.63%），其次为热原体纲（Thermoplasmata，8.51%）古菌。发酵系统中共有22～29个细菌门，其中优势菌群为拟杆菌门（Bacteroidetes，平均含量46.07%）、变形菌门（Proteobacteria，平均含量20.51%）和厚壁菌门（Firmicutes，平均含量13.09%）。玉米秸秆沼气系统微生物群落结构的阐明可为秸秆沼气工程调控提供科学数据。
위연구옥미갈간산소기급기발효과정중미생물군락변화，이예처리후적옥미갈간위원료，채용10 L염양반응기진행비식중온발효산소기。동시취양이용454초린산측서법측정발효과정중미생물군락적변화。결과표명：해계통계동신속，재제3 d취체도산기고봉7.78 L，료용산기솔위0.97 L·L-1·d-1，46 d원료소기산솔화갑완산솔분별위236.84 ml·(g VS)-1화132.23 ml·(g VS)-1。454초린산측서급분석표명계통중고균주요위갑완미균강（Methanomicrobia，점총OTU적89.63%），기차위열원체강（Thermoplasmata，8.51%）고균。발효계통중공유22～29개세균문，기중우세균군위의간균문（Bacteroidetes，평균함량46.07%）、변형균문（Proteobacteria，평균함량20.51%）화후벽균문（Firmicutes，평균함량13.09%）。옥미갈간소기계통미생물군락결구적천명가위갈간소기공정조공제공과학수거。
With pretreated cornstalks as raw material,a mesophilic batch fermentation study using a 10-L anaerobic reactor was carried out to investigate biogas production rate and prokaryotic composition. Fermentation started rapidly, and a peak in biogas production appeared after 3 days with volumetric gas production rate of 0.97 L·L-1·d-1. The rates of biogas production and methane production during 46 days were 236.84 ml·(g VS)-1 and 132.23 ml·(g VS)-1, respectively. During the fermentation process, prokaryotic composition was investigated using pyrosequencing technique. Prokaryotic diversity increased with the fermentation process, and prokaryotic composition shifted dramatically compared to the initial stage. Archaea was dominated by Methanomicrobia (89.63% of total archaeal reads), followed by Thermoplasmata (8.51%). About 22-29 bacterial phyla were identified, including Bacteroidetes (46.07% of total reads), Proteobacteria (20.51%), and Firmicutes (13.09%) as the predominant bacterial communities. Our research provides important implications on the regulation of prokaryotic composition for biogas production using cornstalks.