CAJ | 학술논문

采用非模板软化学法制备了一种孔径均一的介孔TiO2材料(m-TiO2)。XRD测试结果表明其晶型为锐钛矿，且结晶度高，通过N2吸-脱附曲线可看出存在规整的介孔结构且具有高比表面，从FESEM和TEM可以看出制备的m-TiO2由许多纳米粒子构成微米级大颗粒，且存在均匀分布的介孔。将此种TiO2材料固定化葡萄糖氧化酶(GOx)后，通过红外光谱(FT-IR)分析可知m-TiO2材料可以有效固定化GOx。将m-TiO2作为固定化材料固定化GOx制备成Nafion/GOx/m-TiO2/GC电极并进行相关电化学测试，测试结果表明m-TiO2作为固定化材料固定化GOx后具有良好的催化活性，在无电子媒介体存在下能够实现反应电子与电极表面的直接电子传递，检测线性范围为0.1～1.2 mmol·L-1葡萄糖，灵敏度为3.44μA·mmol-1·L·cm-2，在葡萄糖传感领域具有良好的应用前景。
채용비모판연화학법제비료일충공경균일적개공TiO2재료(m-TiO2)。XRD측시결과표명기정형위예태광，차결정도고，통과N2흡-탈부곡선가간출존재규정적개공결구차구유고비표면，종FESEM화TEM가이간출제비적m-TiO2유허다납미입자구성미미급대과립，차존재균균분포적개공。장차충TiO2재료고정화포도당양화매(GOx)후，통과홍외광보(FT-IR)분석가지m-TiO2재료가이유효고정화GOx。장m-TiO2작위고정화재료고정화GOx제비성Nafion/GOx/m-TiO2/GC전겁병진행상관전화학측시，측시결과표명m-TiO2작위고정화재료고정화GOx후구유량호적최화활성，재무전자매개체존재하능구실현반응전자여전겁표면적직접전자전체，검측선성범위위0.1～1.2 mmol·L-1포도당，령민도위3.44μA·mmol-1·L·cm-2，재포도당전감영역구유량호적응용전경。
A novel mesoporous TiO2 material (m-TiO2) was obtained by a soft chemistry method in the absence of any surfactants or templates. It was shown that the m-TiO2 had an anatase crystalline structure with well-distributed mesopores from XRD, N2 adsorption-desorption isotherms, FESEM and TEM. The FT-IR results indicated that glucose oxidase (GOx) could be well immobilized on m-TiO2. The electrochemical tests of the fabricated Nafion/GOx/m-TiO2 modified glass carbon electrode (GCE) showed fast direct electron transfer between GOx molecules and electrode surface, exhibiting a linear response to glucose concentration ranging from 0.1 to 1.2 mmol·L-1, and good sensitivity of 3.44μA·mmol-1·L·cm-2, which proved that the novel m-TiO2 was a promising material for immobilization of GOx and fabrication of glucose biosensors.