CAJ | 학술논문

通过500℃下焙烧H2Ti2O5制备介孔TiO2-500作为高效液相色谱的固定相填料，测试牛血清蛋白（BSA）、肌红蛋白（myoglobin）、溶菌酶（lysozyme）在不同 pH 环境下的停留时间分布，结果表明，在 pH=4.7，三种蛋白质几乎都固定化，在pH=7.4和11条件下，三种蛋白通过色谱柱的停留时间为溶菌酶>肌红蛋白>牛血清蛋白。三种蛋白在介孔TiO2-500表面的宏观常压吸附量的结果与色谱结果相一致，介孔TiO2-500对于三种蛋白的固定化能力为溶菌酶>肌红蛋白>牛血清蛋白。因此，该介孔TiO2对不同蛋白的显著的固定化性能的差异使其在蛋白质的吸附和分离、固定化酶等领域有着潜在的应用。
통과500℃하배소H2Ti2O5제비개공TiO2-500작위고효액상색보적고정상전료，측시우혈청단백（BSA）、기홍단백（myoglobin）、용균매（lysozyme）재불동 pH 배경하적정류시간분포，결과표명，재 pH=4.7，삼충단백질궤호도고정화，재pH=7.4화11조건하，삼충단백통과색보주적정류시간위용균매>기홍단백>우혈청단백。삼충단백재개공TiO2-500표면적굉관상압흡부량적결과여색보결과상일치，개공TiO2-500대우삼충단백적고정화능력위용균매>기홍단백>우혈청단백。인차，해개공TiO2대불동단백적현저적고정화성능적차이사기재단백질적흡부화분리、고정화매등영역유착잠재적응용。
Mesoporous TiO2 with adjustable pore diameter, high specific surface area and excellent biocompatibility has a good application prospect in biological molecules immobilization carriers. Mesoporous TiO2-500 were prepared as the stationary phase packing in High Performance Liquid Chromatograph by calcinating H2Ti2O5 at 500℃, and the TiO2 samples were characterized by X-ray diffraction (XRD), N2 adsorption-desorption and field-emmission scanning electron microscopy (FESEM). The residence timeunder different pH of bovine serum albumin, myoglobin and lysozyme through the new chromatograph column were tested. Nearly all three kinds of proteins were immobilized at pH =4.7, and the order of residence time of three proteins was lysozyme >myoglobin > bovine serum albumin under the condition of pH=7.4 and 11. Adsorption of three kinds of proteins on the surface of the mesoporous TiO2-500 under atmospheric pressure was consistent with chromatographic results, and the order of the immobilization capacity of mesoporous TiO2-500 for three kinds of proteins was lysozyme > myoglobin >bovine serum albumin. Therefore, due to the significant difference in protein immobilization performance, the mesoporous TiO2has a potential application in protein adsorption and separation, immobilizing enzyme and other fields.