CAJ | 학술논문

目的：初步探究清热化痰法指导下的蒌芩止嗽煎对慢性阻塞性肺病急性加重期( AECOPD)大鼠血清中性粒细胞弹性蛋白酶水平的影响。方法：建立大鼠AECOPD模型,60只AECOPD大鼠模型随机分为模型对照组(20只)、氨溴索组(20只)、蒌芩止嗽煎组(20只),用酶联免疫吸附法(ELISA)测定AECOPD大鼠模型治疗前后血清中中性粒细胞弹性蛋白酶(NE)指标。结果：蒌芩止嗽煎组、氨溴索组治疗后与治疗前比较,NE均有明显下降(P<0.01)。结论：清热化痰法指导下的蒌芩止嗽煎初步研究是通过降低NE水平,从而减少AECOPD大鼠模型的气道黏液高分泌,减轻气道阻塞。
목적：초보탐구청열화담법지도하적루금지수전대만성조새성폐병급성가중기( AECOPD)대서혈청중성립세포탄성단백매수평적영향。방법：건립대서AECOPD모형,60지AECOPD대서모형수궤분위모형대조조(20지)、안추색조(20지)、루금지수전조(20지),용매련면역흡부법(ELISA)측정AECOPD대서모형치료전후혈청중중성립세포탄성단백매(NE)지표。결과：루금지수전조、안추색조치료후여치료전비교,NE균유명현하강(P<0.01)。결론：청열화담법지도하적루금지수전초보연구시통과강저NE수평,종이감소AECOPD대서모형적기도점액고분비,감경기도조새。
Objective:To preliminarily study the effect of Louqin Zhisou decoctions with the mechanism of clearing heat and resol-ving phlegm on the blood level of neutrophil elastase ( NE) in the rat model of acute exacerbation of chronic obstructive pulmonary dis-ease ( AECOPD) . Methods:The rat model of AECOPD was established by passive cigarette smoking, intratracheal instillation of li-popolysacchricle ( LPY) and intranasal instillation of staphylococcus aureus. Totally 60 AECOPD rats were randomly divided into 3 groups, the model control group (n=20), ambrohexel group (n=20), and Louqin Zhisou decoctions group (n=20). NE was detec-ted by ELISA. Results:Compared with that before the treatment, NE in ambrohexel group and Louqin Zhisou decoctions group was de-creased significantly(P<0. 01). Conclusion:Clearing heat and resolving phlegm method probably can decrease NE by reducing air-way mucus hypersecretion and obstruction in AECOPD rats.