中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2014年
5期
679-683
,共5页
李敏%孙玲%李红玲%孙黔云
李敏%孫玲%李紅玲%孫黔雲
리민%손령%리홍령%손검운
内皮细胞%氧化型低密度脂蛋白%NF-κB%阿托伐他汀%炎症%黏附分子
內皮細胞%氧化型低密度脂蛋白%NF-κB%阿託伐他汀%炎癥%黏附分子
내피세포%양화형저밀도지단백%NF-κB%아탁벌타정%염증%점부분자
endothelial cell%oxidized low density lip-oprotein%nuclear factor κB%atorvastatin%inflamma-tion%adhesion molecule
目的研究阿托伐他汀( ATV)对氧化型低密度脂蛋白( ox-LDL)导致人微血管内皮细胞活化和损伤的抑制作用。方法采用ox-LDL激活和损伤人微血管内皮细胞。损伤前加入ATV与内皮细胞孵育。细胞暴露于ox-LDL后,MTT法测定细胞活力,酶活性测定法检测培养上清中乳酸脱氢酶( LDH)活力, ELISA 检测上清中 ICAM-1含量, Western blot检测细胞质内NF-κB p65的磷酸化水平,并采用双萤光素酶报告基因检测系统测定NF-κB的核内转录活性。结果 Ox-LDL能导致人微血管内皮细胞的活化和损伤。 ATV的干预可有效减轻ox-LDL对细胞活力的抑制、减少LDH释放、下调ICAM-1分子的表达、减弱细胞质内NF-κB p65的磷酸化、抑制NF-κB核内转录活性的上调。结论 ATV能有效减轻ox-LDL导致的人微血管内皮细胞活化和损伤,其机制可能与抑制NF-κB的磷酸化和核内转录活性有关。
目的研究阿託伐他汀( ATV)對氧化型低密度脂蛋白( ox-LDL)導緻人微血管內皮細胞活化和損傷的抑製作用。方法採用ox-LDL激活和損傷人微血管內皮細胞。損傷前加入ATV與內皮細胞孵育。細胞暴露于ox-LDL後,MTT法測定細胞活力,酶活性測定法檢測培養上清中乳痠脫氫酶( LDH)活力, ELISA 檢測上清中 ICAM-1含量, Western blot檢測細胞質內NF-κB p65的燐痠化水平,併採用雙螢光素酶報告基因檢測繫統測定NF-κB的覈內轉錄活性。結果 Ox-LDL能導緻人微血管內皮細胞的活化和損傷。 ATV的榦預可有效減輕ox-LDL對細胞活力的抑製、減少LDH釋放、下調ICAM-1分子的錶達、減弱細胞質內NF-κB p65的燐痠化、抑製NF-κB覈內轉錄活性的上調。結論 ATV能有效減輕ox-LDL導緻的人微血管內皮細胞活化和損傷,其機製可能與抑製NF-κB的燐痠化和覈內轉錄活性有關。
목적연구아탁벌타정( ATV)대양화형저밀도지단백( ox-LDL)도치인미혈관내피세포활화화손상적억제작용。방법채용ox-LDL격활화손상인미혈관내피세포。손상전가입ATV여내피세포부육。세포폭로우ox-LDL후,MTT법측정세포활력,매활성측정법검측배양상청중유산탈경매( LDH)활력, ELISA 검측상청중 ICAM-1함량, Western blot검측세포질내NF-κB p65적린산화수평,병채용쌍형광소매보고기인검측계통측정NF-κB적핵내전록활성。결과 Ox-LDL능도치인미혈관내피세포적활화화손상。 ATV적간예가유효감경ox-LDL대세포활력적억제、감소LDH석방、하조ICAM-1분자적표체、감약세포질내NF-κB p65적린산화、억제NF-κB핵내전록활성적상조。결론 ATV능유효감경ox-LDL도치적인미혈관내피세포활화화손상,기궤제가능여억제NF-κB적린산화화핵내전록활성유관。
Aim To investigate the effects of atorvas-tatin ( ATV) on activation and injury of microvascular endothelial cells induced by oxidized low density lipo-protein ( ox-LDL) . Methods Cultured human micro-vascular endothelial cells were pre-incubated with ATV for 24 h prior to exposure of endothelial cells to ox-LDL. After exposure of endothelial cells to ox-LDL, the cell viability was measured by MTT method, LDH in supernatants was determined by enzyme activity as-say kit, ICAM-1 in supernatants was assayed by using ELISA method, phosphorylation of NF-κB p65 was de-tected by western blot analysis, transcriptional activity of NF-κB signal pathway was measured by employing dual-luciferase reporter assay system. Results Hu-man microvascular endothelial cells were activated and injured by ox-LDL. Inhibition of the cell viability, re-lease of LDH, expression of ICAM-1, phosphorylation of NF-κB p65 , and up-regulated transcriptional activity of NF-κB induced by ox-LDL were attenuated by ATV. Conclusion ATV can significantly inhibit the activa-tion and injury of human microvascular endothelial cells induced by ox-LDL, and that may be related to inhibition of phosphorylation and transcriptional activity of NF-κB.
