CAJ | 학술논문

目的：观察青刺果总黄酮对四氧嘧啶导致的糖尿病小鼠肾组织形态学的影响，探讨其对肾脏的保护作用。方法腹腔注射四氧嘧啶建立糖尿病小鼠模型，连续4周给予青刺果总黄酮灌胃治疗。2周及4周后分别处死小鼠，测血糖，称肾重和计算肾脏脏器指数。取部分肾做切片，分别在光镜和电镜下观察肾脏病理变化。结果糖尿病模型小鼠经青刺果总黄酮治疗后，在光镜和电镜下均可观察到由四氧嘧啶导致的肾脏病变的减轻。经青刺果总黄酮治疗的糖尿病小鼠其肾小球体积减小，基底膜无明显增厚；肾小管内糖原沉积减少，间质纤维减少；细胞器损伤较模型组轻微。结论青刺果总黄酮能有效的改善糖尿病小鼠肾脏的病变，控制肾脏肥大，对糖尿病肾病有一定的保护作用。
목적：관찰청자과총황동대사양밀정도치적당뇨병소서신조직형태학적영향，탐토기대신장적보호작용。방법복강주사사양밀정건립당뇨병소서모형，련속4주급여청자과총황동관위치료。2주급4주후분별처사소서，측혈당，칭신중화계산신장장기지수。취부분신주절편，분별재광경화전경하관찰신장병리변화。결과당뇨병모형소서경청자과총황동치료후，재광경화전경하균가관찰도유사양밀정도치적신장병변적감경。경청자과총황동치료적당뇨병소서기신소구체적감소，기저막무명현증후；신소관내당원침적감소，간질섬유감소；세포기손상교모형조경미。결론청자과총황동능유효적개선당뇨병소서신장적병변，공제신장비대，대당뇨병신병유일정적보호작용。
Aim To investigate the effect of flavonoids from Prinsepia utilis Royle( FPR) on the histomorphol-ogy of kidney in diabetic mice, and to investigate its protective mechanism. Methods Diabetic mice in-duced by alloxan were given FPR orally each day for four weeks. After the administration for two and four weeks, ten mice in each group were randomly sacri-ficed. The kidneys were removed and weighed. The extracted renal tissue was embedded with paraffin and sectioned, the sections were stained with Hematoxylin and Eosin(HE)、Periodic acid Schiff(PAS) and Go-mori, and then observed under the microscopy. 1mm3 of renal cortex fixed with glutaral in four centi-degree , and then the ultrastructure of each group was observed under the electron microscope respectively after four weeks′ treatment. Results Compared with the model control group, in the treatment group, observation un-der the microscopy showed that glomerular volume and mesangial cells reduced, FPR could relieve thickening of the glomerular basement membrane ( GBM ) , little inflammatory cells infiltrated in the interstitium,tubular epithelial cells almost became normal, renal tubule had little glucogen, fiber decreased in the interstitium of renal tubule. Observation under the electron micro-scope indicated that foot process in podocytes lined up in order, mitochondria of the renal tubule’ s epithelial cell almost recovered. Conclusion FPR can relieve the changes of renal pathology,improve renal function, and delay the progression of pathologic changes of kid-ney in diabetic mice partly through reducing the blood glucose and the blood lipid.