CAJ | 학술논문

目的：研究8-溴-7-甲氧基白杨素(8-bromo-7-methoxy-chrysin, BrMC)能否有效逆转人宫颈癌SiHa细胞系球形成细胞上皮-间叶样表型转化( epithelial-mesenchymal transi-tion, EMT)。方法体外培养SiHa细胞系细胞,干细胞条件培养基悬浮培养得到球形成细胞( sphere forming cells, SFCs)。以BrMC处理SFCs后,通过单层贴壁培养观察细胞形态。 Western blot 分析 SFCs 的 E-cadherin、N-cadherin 和Twist1蛋白表达水平。结果与亲本细胞比较,SiHa细胞系SFCs呈现间叶样细胞(梭形细胞)形态,并低表达E-cadherin和高表达N-cadherin蛋白。 BrMC(1．0μmol·L-1)处理72 h后,SFCs由梭形细胞转变为多边形细胞；BrMC(0．1和1．0μmol·L-1)作用24 h,SFCs中E-cadherin表达上调,N-cad-herin和Twist1蛋白表达降低。结论 BrMC具有逆转SiHa细胞系SFCs的 EMT作用,其机制涉及降低 Twsit1蛋白表达。
목적：연구8-추-7-갑양기백양소(8-bromo-7-methoxy-chrysin, BrMC)능부유효역전인궁경암SiHa세포계구형성세포상피-간협양표형전화( epithelial-mesenchymal transi-tion, EMT)。방법체외배양SiHa세포계세포,간세포조건배양기현부배양득도구형성세포( sphere forming cells, SFCs)。이BrMC처리SFCs후,통과단층첩벽배양관찰세포형태。 Western blot 분석 SFCs 적 E-cadherin、N-cadherin 화Twist1단백표체수평。결과여친본세포비교,SiHa세포계SFCs정현간협양세포(사형세포)형태,병저표체E-cadherin화고표체N-cadherin단백。 BrMC(1．0μmol·L-1)처리72 h후,SFCs유사형세포전변위다변형세포；BrMC(0．1화1．0μmol·L-1)작용24 h,SFCs중E-cadherin표체상조,N-cad-herin화Twist1단백표체강저。결론 BrMC구유역전SiHa세포계SFCs적 EMT작용,기궤제섭급강저 Twsit1단백표체。
Aim To investigate whether 8-bromo-7-methoxychrysin ( BrMC ) can reverse the epithelial-mesenchymal transition ( EMT) in sphere-forming cells (SFCs) derived from cervical cancer SiHa cell line. Methods Human cervical cancer SiHa cell line was cultured in vitro and then SFCs were obtained from Si-Ha cell line using suspension culture with the stem-condition culture system. After treatment with BrMC for 72 h, the morphology of SFCs of SiHa cell line was observed via monolayer adherence culture. The expres-sion levels of E-cadherin, N-cadherin and Twist1 pro-teins were analyzed using western blot assay. Results SFCs of SiHa cell line presented the mesenchyme-like( spindle-shaped) cell shape and exhibited reduced E-cadherin and elevated N-cadherin protein expres-sion, compared with the parental cells. After treatment with BrMC(1. 0 μmol·L-1 ) for 72 h, SFCs of SiHa cell line changed from spindle-shaped cells in to poly-gon shaped cells under monolayer adherence culture. After treatment with BrMC for 24 h, the expression lev-els of E-cadherin protein were up-regulated and the ex-pression levels of N-cadherin and Twist1 proteins were decreased in SFCs derived from SiHa cell line. Con-clusion BrMC effectively reverses EMT of SFCs de-rived from cervical cancer SiHa cell line, and its mechanism is involved in down-regulating the expres-sion of Twist protein.