CAJ | 학술논문

目的：研究松果菊苷( ECH)对血管性痴呆大鼠氧化应激损伤的保护作用。方法采用间隔3d分2次结扎大鼠双侧颈总动脉法制备血管性痴呆( VD)模型；给药后,采用生化方法测定皮层及海马组织中还原型谷胱甘肽( GSH)、一氧化氮( NO)含量,谷胱甘肽过氧化物酶( GSH-Px)、一氧化氮合酶( NOS)的活力；HE染色,光学显微镜下观察各组大鼠海马组织CA1区组织结构的变化。结果与假手术组比较,模型组大鼠的皮层及海马组织中的GSH 含量以及GSH-Px的活力明显下降(P<0．01),NO含量及NOS的活力明显升高(P<0．05, P<0．01),海马CA1区细胞数目减少,细胞排列紊乱稀疏,细胞形态不完整,结构不正常,胞质稀少,胞核与胞质界限模糊,细胞核深染、固缩,呈三角形或不规则形,核仁不明显,并出现增生胶质细胞；与模型组比较,各给药组大鼠皮层及海马组织中的GSH 含量以及GSH- Px的活力有不同程度的升高(P<0．05, P<0．01),NOS的活力有不同程度的降低(P<0．05, P<0．01),同时大鼠海马CA1区神经元排列较为整齐,结构相对正常,核固缩现象减少,深染程度降低；与阳性药组以及假手术组比较,ECH高剂量组的皮层及海马组织中 GSH、NO含量, GSH-Px、NOS的活力差异均无显著性( P>0．05),且ECH高剂量组海马CA1区神经元损伤程度低,更接近于假手术组。结论松果菊苷对血管性痴呆大鼠氧化应激损伤有较好的保护作用。
목적：연구송과국감( ECH)대혈관성치태대서양화응격손상적보호작용。방법채용간격3d분2차결찰대서쌍측경총동맥법제비혈관성치태( VD)모형；급약후,채용생화방법측정피층급해마조직중환원형곡광감태( GSH)、일양화담( NO)함량,곡광감태과양화물매( GSH-Px)、일양화담합매( NOS)적활력；HE염색,광학현미경하관찰각조대서해마조직CA1구조직결구적변화。결과여가수술조비교,모형조대서적피층급해마조직중적GSH 함량이급GSH-Px적활력명현하강(P<0．01),NO함량급NOS적활력명현승고(P<0．05, P<0．01),해마CA1구세포수목감소,세포배렬문란희소,세포형태불완정,결구불정상,포질희소,포핵여포질계한모호,세포핵심염、고축,정삼각형혹불규칙형,핵인불명현,병출현증생효질세포；여모형조비교,각급약조대서피층급해마조직중적GSH 함량이급GSH- Px적활력유불동정도적승고(P<0．05, P<0．01),NOS적활력유불동정도적강저(P<0．05, P<0．01),동시대서해마CA1구신경원배렬교위정제,결구상대정상,핵고축현상감소,심염정도강저；여양성약조이급가수술조비교,ECH고제량조적피층급해마조직중 GSH、NO함량, GSH-Px、NOS적활력차이균무현저성( P>0．05),차ECH고제량조해마CA1구신경원손상정도저,경접근우가수술조。결론송과국감대혈관성치태대서양화응격손상유교호적보호작용。
Aim To investigate the protective effects of echinacoside ( ECH ) on oxidative stress injury in vascular dementia rats. Methods Vascular dementia model was duplicated by means of permanent ligation of bilateral common carotid artery at two times intervals for three days. Biochemical methods was used to detect the GSH, NO, GSH-Px, NOS in each group rat’ s cor-tex and hippocampus. The change of the tissue struc-ture in CA1 area of hippocampus in each group was ob-served and analyzed by microscope after HE staining. Results Compared with sham group, the content of GSH and activity of GSH-Px in the rats of the model group were decreased significantly ( P<0. 01 ) , while the content of NO and activity of NOS were increased obviously (P<0. 05, P <0. 01). Meanwhile, in tis-sue structure of rat’ s hippocampal CA1 area, the num-bers of cells were reduced, cell arrangement was sparse and in disorder, cell form was not intact and cell struc-ture was not normal, cytoplasm, nucleus and cyto-plasm were blurry, nuclei were hyperchromatic and in contraction, and were changed to triangular or irregular shape, lacking nucleoli, and proliferation of glial cells appeared. Compared with the model group, the content of GSH and activity of GSH-Px in the rats of the treat-ment groups were increased significantly ( P<0. 05 , P<0 . 01 ) , while the activity of NOS was decreased ob-viously (P<0. 05, P<0. 01). Meanwhile, in rat tis-sue structure of hippocampal CA1 area, the rat cells in each treatment group were arranged in order, the form of structure was normal, the nuclei were less hyper-chromatic and contracted. Compared with Gal group and sham group, the rats of ECH high dosage group were not different in every part of experiment ( P >0. 05 ) . Conclusion ECH exerts protection on oxida-tive stress injury in vascular dementia rats.