CAJ | 학술논문

微生物是气溶胶的重要组成部分。本研究通过收集南极长城站附近气溶胶，构建气溶胶中空气微生物16S rRNA基因文库，发现南极空气微生物具有很高的多样性，93个克隆子包含了53个OTU（Operational Taxonomic Unit）， Shannon指数为3．58，Simpson指数为0．04。南极长城站空气微生物的主要细菌类群为Actinobacteria、Cyanobacteria/Chloroplasts、Bacteroidetes、Firmicutes、Planctomycetes、Proteobacteria及Verrucomicrobia等7个门，Proteobacteria门的细菌是优势类群，占长城站空气微生物细菌克隆文库的65．7％。克隆文库中海源微生物序列及陆源微生物序列各占30．1％，说明长城站附近具有较强的海陆大气交换。我们发现有15个克隆子序列与人类活动相关，占总克隆子数的16．1％，说明人类活动已经影响到了长城站的空气微生物群落结构。
미생물시기용효적중요조성부분。본연구통과수집남겁장성참부근기용효，구건기용효중공기미생물16S rRNA기인문고，발현남겁공기미생물구유흔고적다양성，93개극륭자포함료53개OTU（Operational Taxonomic Unit）， Shannon지수위3．58，Simpson지수위0．04。남겁장성참공기미생물적주요세균류군위Actinobacteria、Cyanobacteria/Chloroplasts、Bacteroidetes、Firmicutes、Planctomycetes、Proteobacteria급Verrucomicrobia등7개문，Proteobacteria문적세균시우세류군，점장성참공기미생물세균극륭문고적65．7％。극륭문고중해원미생물서렬급륙원미생물서렬각점30．1％，설명장성참부근구유교강적해륙대기교환。아문발현유15개극륭자서렬여인류활동상관，점총극륭자수적16．1％，설명인류활동이경영향도료장성참적공기미생물군락결구。
Bacteria are an important component of aerosols,which are closely connected with human life.This study ana-lyzed the community structure of air-borne bacteria at the Great Wall Station,Antarctica,based on 1 6S rRNA gene clone library analysis.High diversity of airborne bacteria was observed in the aerosol samples.Ninety-three se-quences from the clone library could be classified into 53 OTUs (Operation Taxonomic Units)based on 97% se-quence similarity.The Shannon and Simpson index of the clone library were 3.58 and 0.04,respectively.Based on phylogenetic analysis the sequences were affiliated with seven phyla,including Actinobacteria,Cyanobacteria/Chloroplast,Bacteroidetes,Firmicutes,Planctomycetes,Proteobacteria and Verrucomicrobia.Proteobacterial se-quences accounted for 65 .7%of the clone library.Sequences derived from marine and terrestrial environments ac-counted for 30.1%of the library,suggesting a strong atmospheric exchange between land and sea at the Great Wall Station.In addition,there were 1 5 sequences related to human activity,indicating that human influence has al-ready affected the airborne microbial community structure at the Great Wall Station.