华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
2期
110-115
,共6页
谢欢%杨文香%彭巧慧%赵丽娟%刘力伟%张娜%刘大群
謝歡%楊文香%彭巧慧%趙麗娟%劉力偉%張娜%劉大群
사환%양문향%팽교혜%조려연%류력위%장나%류대군
小麦叶锈病%抗病基因%MITE%分子标记
小麥葉鏽病%抗病基因%MITE%分子標記
소맥협수병%항병기인%MITE%분자표기
Wheat leaf rust%Resistance gene%MITE%Molecular markers
以Thatcher及23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系为材料，开展了MITE扩增体系的建立研究，获得了适合小麦MITE分子标记的扩增体系及不同引物的退火温度，所用56对引物有27对获得稳定扩增。
以Thatcher及23箇以Thatcher為遺傳揹景的小麥抗葉鏽病近等基因繫為材料，開展瞭MITE擴增體繫的建立研究，穫得瞭適閤小麥MITE分子標記的擴增體繫及不同引物的退火溫度，所用56對引物有27對穫得穩定擴增。
이Thatcher급23개이Thatcher위유전배경적소맥항협수병근등기인계위재료，개전료MITE확증체계적건립연구，획득료괄합소맥MITE분자표기적확증체계급불동인물적퇴화온도，소용56대인물유27대획득은정확증。
Thatcher and 23 of wheat leaf rust resistance near isogenic lines in Thatcher background were chosen as materials in this experiment. Research on the establishment of MITE amplification system was carried out. 27 of the 56 primer pairs were amplified stably,reaction system and different annealing temperature of primers were con-firmed for amplification of MITE ( Miniature inverted repeat transposable elements) .
