CAJ | 학술논문

旨在检测以小黑麦干酒糟及其可溶物( TDDGS)替代肉牛日粮中的所有粗料和部分精料对5种瘤胃代表性菌( Fibrobacter succinogenes、Succinovibrio dextrinosolvens、Selenomonas ruminantium、Megasphaera elsdenii 和 Prevotella ru-minicola)比例的影响。利用Real-time PCR法检测了饲喂TDDGS前后5种瘤胃代表性菌数量百分比的变化。结果表明,30% TDDGS组与对照组相比,瘤胃中F. succinogenes、S. ruminantium和M. elsdenii菌数量百分比均有升高,分别显著升高3倍(P﹤0.05)、10倍(P﹤0.05)和2倍(P﹤0.05),而S. dextrinosolvens和P. ruminicola菌数量百分比却都降低了,但差异均不显著。
지재검측이소흑맥간주조급기가용물( TDDGS)체대육우일량중적소유조료화부분정료대5충류위대표성균( Fibrobacter succinogenes、Succinovibrio dextrinosolvens、Selenomonas ruminantium、Megasphaera elsdenii 화 Prevotella ru-minicola)비례적영향。이용Real-time PCR법검측료사위TDDGS전후5충류위대표성균수량백분비적변화。결과표명,30% TDDGS조여대조조상비,류위중F. succinogenes、S. ruminantium화M. elsdenii균수량백분비균유승고,분별현저승고3배(P﹤0.05)、10배(P﹤0.05)화2배(P﹤0.05),이S. dextrinosolvens화P. ruminicola균수량백분비각도강저료,단차이균불현저。
The aim of this study was to explore the effects of triticale dried distillers grains with solubles ( TD-DGS) incorporation in beef cattle diets on the relative abundance of five rumen bacteria viz. ,Fibrobacter succino-genes,Succinovibrio dextrinosolvens,Selenomonas ruminantium,Megasphaera elsdenii and Prevotella ruminicola. Real-time PCR was used to detect the gene expression of these five rumen bacteria. As compared with control group,the results showed that the relative abundance of F. succinogenes,S. ruminantium and M. elsdenii in 30% TDDGS group increased,and a 3-fold (P<0. 05),a 10-fold (P<0. 05) and a 2-fold (P<0. 05) significant increase were ob-served respectively,however,the relative abundance of S. dextrinosolvens and P. ruminicola decreased in 30% TD-DGS group but no significant differences were found.