CAJ | 학술논문

通过前期对江苏水稻地方品种抗条纹叶枯病资源的筛选获得的一份高抗条纹叶枯病水稻品种旱稻,配制其与条纹叶枯病感病品种武育粳3号杂交组合,采用田间自然接种鉴定方法,以病情指数为表型值,对141个F2单株的F2：3株系进行了条纹叶枯病抗性鉴定。同时利用群体分离分析法( Bulked segregant analysis,BSA法)对抗/感DNA池分析发现第11染色体SSR标记RM209、RM21与条纹叶枯病抗性有明显的连锁关系。利用基于完备复合区间作图方法的QTL检测软件(QTL IciMapping V3.2)在第11染色体上检测到一个条纹叶枯病抗性相关QTL,位于SSR标记RM209和RM21之间,LOD值为13.25,可解释表型变异38.39%,加性效应为负值,表明该数量性状基因座对水稻条纹叶枯病的抗性来自抗病亲本旱稻。
통과전기대강소수도지방품충항조문협고병자원적사선획득적일빈고항조문협고병수도품충한도,배제기여조문협고병감병품충무육갱3호잡교조합,채용전간자연접충감정방법,이병정지수위표형치,대141개F2단주적F2：3주계진행료조문협고병항성감정。동시이용군체분리분석법( Bulked segregant analysis,BSA법)대항/감DNA지분석발현제11염색체SSR표기RM209、RM21여조문협고병항성유명현적련쇄관계。이용기우완비복합구간작도방법적QTL검측연건(QTL IciMapping V3.2)재제11염색체상검측도일개조문협고병항성상관QTL,위우SSR표기RM209화RM21지간,LOD치위13.25,가해석표형변이38.39%,가성효응위부치,표명해수량성상기인좌대수도조문협고병적항성래자항병친본한도。
The Jiangsu landrace rice variety Handao was previously identified as high resistance to rice stripe disease (RSV). A total of 141 F2:3 lines derived from the cross of Handao and Wuyujing 3,a variety high sensitive to RSV,were investigated by natural infection method in field,and the disease rating index was scored. Analysis of the resistant/sensitive pools by bulked segregant analysis indicated that the SSR markers RM209 and RM21 on rice chromosome 11 obviously linked to RSV resistance. A QTL for RSV was detected on rice chromosome 11 between RM209 and RM21 by the software QTL IciMapping V3 . 2 based on the principle of inclusive composite interval mapping (ICIM) with the maximum LOD score 13. 25,which explained 38. 39% of the trait variance. Additive effects of qSTV11HD was negative,indicating that the resistance allele was inherited from Handao.