CAJ | 학술논문

采用RT-PCR方法,分段扩增出猪繁殖与呼吸综合征病毒( PRRSV) Shanxi-6株15个基因片段,并将这15个片段分别连接到pMD18-T载体上进行克隆测序、拼接后,得到PRRSV Shanxi-6株全基因组长15320 bp (不包含PolyA尾),包含9个开放阅读框,5′UTR长189 bp,3′UTR长150 bp。基因组序列分析结果表明,Shanxi-6株与欧洲型代表毒株LV的核苷酸序列同源性为54.35%,与美洲型代表毒株VR-2332的核苷酸序列同源性为88.80%,与HP-PRRSV毒株(JXA1、HUN4、HuN、HUB1)亲缘关系最近,核苷酸序列同源性高达97.44%~97.74%,且在非结构蛋白Nsp2上有30个氨基酸的缺失。
채용RT-PCR방법,분단확증출저번식여호흡종합정병독( PRRSV) Shanxi-6주15개기인편단,병장저15개편단분별련접도pMD18-T재체상진행극륭측서、병접후,득도PRRSV Shanxi-6주전기인조장15320 bp (불포함PolyA미),포함9개개방열독광,5′UTR장189 bp,3′UTR장150 bp。기인조서렬분석결과표명,Shanxi-6주여구주형대표독주LV적핵감산서렬동원성위54.35%,여미주형대표독주VR-2332적핵감산서렬동원성위88.80%,여HP-PRRSV독주(JXA1、HUN4、HuN、HUB1)친연관계최근,핵감산서렬동원성고체97.44%~97.74%,차재비결구단백Nsp2상유30개안기산적결실。
The fifteen gene fragments of Shanxi-6 strain of porcine reproductive and respiratory syndrome were amplified respectively by RT-PCR,cloned into pMD18-T vector and sequenced. The genome of Shanxi-6 strain in length was 15 320 bp excluding PloyA tail,containing 9 open reading frames ( ORFs) ,the 5′UTR in 189 bp and the 3′UTR in 150 bp. The complete genomic analysis showed that Shanxi-6 strain shared 54. 35% of nucleotide ho-mology with European genotype (LV) and shared 88. 80% homology with American genotype (VR-2332). Shanxi-6 strain has correlative genetic relationship and shared 97 . 44% -97 . 74% homology with highly pathogenic PRRSV (JXA1,HUN4,HuN,HUB1). 30 amino acids deletion was exhibited in Nsp2 protein of Shanxi-6 strain.