华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
2期
56-61
,共6页
王竹林%李美霞%奚亚军%刘曙东
王竹林%李美霞%奚亞軍%劉曙東
왕죽림%리미하%해아군%류서동
小麦%白粉病%Pm4b%抗病鉴定
小麥%白粉病%Pm4b%抗病鑒定
소맥%백분병%Pm4b%항병감정
Wheat%Powdery mildew%Pm4 b%Appraisal of resistance
采用室内苗期鉴定、大田人工接种鉴定和Pm4b分子标记对黄淮麦区的98个小麦品种(系)进行了白粉病抗性分析。苗期鉴定结果表明,被测品种中,抗病品种5个,占参试品种的5%。田间接种鉴定发现,抗病品种39个,占参试品种的39%。分子标记检测发现,98份供试材料中有3份样品检测到Pm4b基因的标记片段,占供试材料的3%。苗期感病而在成株期表现中等抗病有可能是慢病性品种,是一种更有利用前途的抗病种质。
採用室內苗期鑒定、大田人工接種鑒定和Pm4b分子標記對黃淮麥區的98箇小麥品種(繫)進行瞭白粉病抗性分析。苗期鑒定結果錶明,被測品種中,抗病品種5箇,佔參試品種的5%。田間接種鑒定髮現,抗病品種39箇,佔參試品種的39%。分子標記檢測髮現,98份供試材料中有3份樣品檢測到Pm4b基因的標記片段,佔供試材料的3%。苗期感病而在成株期錶現中等抗病有可能是慢病性品種,是一種更有利用前途的抗病種質。
채용실내묘기감정、대전인공접충감정화Pm4b분자표기대황회맥구적98개소맥품충(계)진행료백분병항성분석。묘기감정결과표명,피측품충중,항병품충5개,점삼시품충적5%。전간접충감정발현,항병품충39개,점삼시품충적39%。분자표기검측발현,98빈공시재료중유3빈양품검측도Pm4b기인적표기편단,점공시재료적3%。묘기감병이재성주기표현중등항병유가능시만병성품충,시일충경유이용전도적항병충질。
In this study,the resistance to powdery mildew of 98 wheat varieties ( lines) of Huanghuai wheat growth area was analyzed using seedling identification,artificial inoculation identification in the field and molecular marker analysis of Pm4b. There only 5% tested varieties had resistance to powdery mildew by seedling stage identi-fication. Field inoculation found 39 resistant varieties,accounting for 39% of the tested varieties. There were 3 sam-ples,accounting for 3% of the tested materials,detected the gene marker fragment of Pm4b by molecular markers detected. These varieties which were susceptible in the seeding but disease-resistant in the adult stage were potential adult-plant resistance variety which was more promising in future disease-resistant breeding. This study provides an important basis for powdery mildew resistance breeding of wheat.