CAJ | 학술논문

在添加不同浓度(0、3、6、9、12、15 mmol/L)氯化钙的 MS 培养基上，研究了钙对马铃薯栽培品种“新大坪”试管苗的生长和生理指标的影响.结果表明：培养基无钙时，试管苗生长势很弱，其株高、有效茎节数、叶片数、干鲜质量、可溶性糖和可溶性蛋白含量均较3 mmol/L 处理(MS正常钙用量)大幅下降.当 MS培养基中添加不同浓度的氯化钙后，上述指标均呈先升高后下降的变化趋势，其中以9 mmol/L 处理的各项指标表现最佳.MS 培养基中添加9 mmol/L氯化钙是马铃薯栽培品种“新大坪”试管苗生长的最佳钙浓度.
재첨가불동농도(0、3、6、9、12、15 mmol/L)록화개적 MS 배양기상，연구료개대마령서재배품충“신대평”시관묘적생장화생리지표적영향.결과표명：배양기무개시，시관묘생장세흔약，기주고、유효경절수、협편수、간선질량、가용성당화가용성단백함량균교3 mmol/L 처리(MS정상개용량)대폭하강.당 MS배양기중첨가불동농도적록화개후，상술지표균정선승고후하강적변화추세，기중이9 mmol/L 처리적각항지표표현최가.MS 배양기중첨가9 mmol/L록화개시마령서재배품충“신대평”시관묘생장적최가개농도.
The growth and physiological indexes of potato test-tube plantlet (cv.‘Xindaping’)were studied by using calcium chloride (Ca)at different concentrations(0,3,6,9,12 and 15 mmol/L)in MS me-dium.The results showed that Ca free medium made plantlets growth vigor weak,plant height,number of functional nodes,leaf number,fresh and dry weight,soluble sugar and protein content dropped sharply than 3 mmol treatment (MS standard Ca concentration).When MS culture medium added different concentra-tion of calcium chloride,the above indexes showed a trend of first increase then decrease,all indexes of treatment at 9 mmol/L performed optimum.The results suggested that MS medium added 9 mmol/L calci-um chloride is optimum for potato test-tube plantlets(cv.‘Xindaping’)growth.