CAJ | 학술논문

利用 RT-PCR技术从马铃薯普通栽培品种‘陇薯3号’试管苗根部扩增得到StSnRK2.4基因的 cDNA序列,并对其编码蛋白进行特性和结构分析.结果表明：该基因序列全长1083 bp,编码360个氨基酸,与烟草SnRK2家族基因具有较高的同源性,达95.56%,已注册该基因到 GenBank(No.JX280914)；该蛋白分子量为41.49 kU,理论等电点为5.52,是一个不跨膜的膜内蛋白,主要存在于细胞核中,含有依赖 cAMP/cGMP 蛋白激酶磷酸化、蛋白激酶C磷酸化、酪蛋白激酶Ⅱ磷酸化、酪氨酸蛋白激酶磷酸化和豆蔻酰化位点,丝氨酸/苏氨酸蛋白激酶活性位点信号序列和蛋白激酶 ATP结合区域信号序列,推测该基因功能与植物抗逆境胁迫有关.
이용 RT-PCR기술종마령서보통재배품충‘롱서3호’시관묘근부확증득도StSnRK2.4기인적 cDNA서렬,병대기편마단백진행특성화결구분석.결과표명：해기인서렬전장1083 bp,편마360개안기산,여연초SnRK2가족기인구유교고적동원성,체95.56%,이주책해기인도 GenBank(No.JX280914)；해단백분자량위41.49 kU,이론등전점위5.52,시일개불과막적막내단백,주요존재우세포핵중,함유의뢰 cAMP/cGMP 단백격매린산화、단백격매C린산화、락단백격매Ⅱ린산화、락안산단백격매린산화화두구선화위점,사안산/소안산단백격매활성위점신호서렬화단백격매 ATP결합구역신호서렬,추측해기인공능여식물항역경협박유관.
The cDNA sequence of StSnRK2.4 were cloned by RT-PCR from the test-tube seedling roots of common potato cultivars ‘Longshu-3’.The full sequence contained 1 083 bp and encoded 360 ami-no acids,and there were high homologies with the SnRK2 gene family of tobacco,and the homology was 95.56%.The gene was registered to GenBank (No.JX280914).The bioinformatics analysis showed that the molecular weight of SnRK2.4 was 41.49 kU,the theoretical isoelectric point was 5.52,and it had not trans-membrane domain and mainly existed in the cell nucleus containing some active sites,such as cAMP-and cGMP-dependent protein kinase phosphorylation site,Protein kinase C phosphorylation site,Casein ki-nase II phosphorylation site,Tyrosine kinase phosphorylation site and N-myristoylation site,Serine/Threo-nine protein kinases active-site and protein kinases ATP-binding region.It is suggested that the function of the gene can associate with plant resistance to adversity stress.