CAJ | 학술논문

目的：观察塞来昔布环氧合酶-2（ COX-2）选择性抑制剂引起糖尿病大鼠视网膜组织促红细胞生成素的改变，探讨其防治早期糖尿病视网膜的机制。方法40只SD雄性大鼠随机分为4组，即正常组、糖尿病组、对照组、实验组，每组10只。1％链尿佐菌素腹腔注射建立糖尿病模型，饲养3个月后，处死大鼠取视网膜，HE染色观察视网膜形态学变化，SYBR荧光实时定量PCR检测视网膜组织中COX-2、EPO mRNA表达变化。结果3个月后，HE染色糖尿病组和对照组结构出现了改变；COX-2、EPO mRNA在糖尿病组和对照组，组间两两比较，无统计学差异（ P ＜0．05），其余各组间比较均有显著性差异（ P ＞0．05）。结论塞来昔布能够有效促进EPO的表达，可以用于早期糖尿病视网膜病变防治。
목적：관찰새래석포배양합매-2（ COX-2）선택성억제제인기당뇨병대서시망막조직촉홍세포생성소적개변，탐토기방치조기당뇨병시망막적궤제。방법40지SD웅성대서수궤분위4조，즉정상조、당뇨병조、대조조、실험조，매조10지。1％련뇨좌균소복강주사건립당뇨병모형，사양3개월후，처사대서취시망막，HE염색관찰시망막형태학변화，SYBR형광실시정량PCR검측시망막조직중COX-2、EPO mRNA표체변화。결과3개월후，HE염색당뇨병조화대조조결구출현료개변；COX-2、EPO mRNA재당뇨병조화대조조，조간량량비교，무통계학차이（ P ＜0．05），기여각조간비교균유현저성차이（ P ＞0．05）。결론새래석포능구유효촉진EPO적표체，가이용우조기당뇨병시망막병변방치。
Objective To observe the effect of cyclooxygenase-2 inhibitor on the expression of erythropoietin ( EPO) in diabetic rats retina and the effect of celecoxib on prevention of early diabetic retinopathy .Methods Forty SD male rats were randomly divided into four groups:the normal group , diabetes group , control group and experimental group , 10 in each group.Diabetes model was established by intraperitoneal injection of streptozotocin (STZ) (50 mg/kg).All of the rats were sacrificed at 3 months.Retinae were HE stained to observe morphological changes , and SYBR real-time fluo-rescent quantitative PCR was used to detect COX-2 and EPO mRNA expression changes .Results After 3 months, HE staining showed structural changes in diabetes group and control group .There was no difference in COX-2 or EPO mRNA expression between diabetes group and the control group ( P <0.05), but between diabetes group and other two groups ( P <0.05).Conclusions Celecoxib could effectively promote the expression of EPO , thus can be used for early preven-tion and treatment for diabetic retinopathy .