CAJ | 학술논문

目的：对已分离出来的5株黄芪内生真菌，进行微量提取物、菌液和菌体处理后的抑菌试验。方法：单因素考查与正交试验方法筛选最佳组合；以相对抑制率体现抑菌效果。结果：微量提取物抗大肠杆菌的最佳组合为50％乙醇0％、提取20 min、40倍体积提取液、提取2次；抗金黄色葡萄球菌为最佳组合为70％乙醇、提取10 min、20倍体积提取液、提取3次。菌液浓缩和菌体乙醇提取物的抑菌试验得到菌液浓缩液对大肠杆菌和金黄色葡萄球菌的抑菌率相对较高。结论：本研究确定了黄芪内生真菌抑菌活性物质微量提取的最佳组合；菌液提取物对大肠杆菌与金黄色葡萄球菌有明显的抑菌作用。
목적：대이분리출래적5주황기내생진균，진행미량제취물、균액화균체처리후적억균시험。방법：단인소고사여정교시험방법사선최가조합；이상대억제솔체현억균효과。결과：미량제취물항대장간균적최가조합위50％을순0％、제취20 min、40배체적제취액、제취2차；항금황색포도구균위최가조합위70％을순、제취10 min、20배체적제취액、제취3차。균액농축화균체을순제취물적억균시험득도균액농축액대대장간균화금황색포도구균적억균솔상대교고。결론：본연구학정료황기내생진균억균활성물질미량제취적최가조합；균액제취물대대장간균여금황색포도구균유명현적억균작용。
Objective:For the isolated endophytic fungi of five strains from astragalus , trace extracts, fungi and bacteria processing to the bacteriostatic experiment .Methods: Single factor test and orthogonal experiment method were for screening the best combination in order that relative inhibition rate reflected bacteriostatic effect .Results:Micro extrac-tion of orthogonal design to get the best combination against Escherichia coli was 50%ethanol concentration , extraction time extracting for 2 times, 40 times the volume of 20min extract;Anti Staphylococcus aureus was the best combination of 70%ethanol, extracting 10min, 20 times volume of extract, extract 3 times.This study determines the endophytic fungi of astragalus trace extraction of the optimal combination of antimicrobial active substances .Microbial extract had obvious bacteriostasison to escherichia coli and staphylococcus aureus .Conclusion:This study determines the endophytic fungi of astragalus trace extraction of the optimal combination of antimicrobial active substances .Bacteria liquid extract has an obvious bacteriostasis against Escherichia coli and staphylococcus aureus .