CAJ | 학술논문

本试验旨在研究蛋白酶对8～21日龄肉仔鸡生长性能、氨基酸表观消化率及肠道组织形态的影响。选择7日龄体重相近、健康的爱拔益加（ AA）肉仔鸡560只（公母各占1/2），随机分为5个组，每组8个重复，每个重复14只鸡。对照组饲喂玉米-豆粕-肉骨粉型基础饲粮，试验组分别饲喂在基础饲粮中添加0．01％、0．02％、0．04％和0．08％蛋白酶的试验饲粮，试验期14 d。结果表明：1）各蛋白酶组肉仔鸡平均日采食量显著高于对照组（P＜0．05），0．02％蛋白酶组肉仔鸡平均日增重显著高于对照组（ P＜0．05）。2）蛋白酶可二次性提高肉仔鸡粗蛋白质和氨基酸（亮氨酸和异亮氨酸除外）的表观消化率（ P＜0．05），0．02％蛋白酶组肉仔鸡粗蛋白质表观消化率显著高于对照组（ P＜0．05），0．01％和0．02％蛋白酶组天冬氨酸、苏氨酸、丝氨酸和丙氨酸的表观消化率均显著高于对照组（ P＜0．05）。3）随蛋白酶添加水平增加，十二指肠隐窝深度呈显著线性升高（P＜0．05），绒毛高度/隐窝深度呈显著线性降低（P＜0．05）；空肠绒毛高度呈显著二次曲线升高（P＜0．05），隐窝深度呈显著线性升高（P＜0．05）。由此可见，玉米-豆粕-肉骨粉型饲粮添加0．01％和0．02％蛋白酶能够促进肉仔鸡生长前期的小肠发育，提高粗蛋白质及大部分氨基酸表观消化率，从而改善生长性能。
본시험지재연구단백매대8～21일령육자계생장성능、안기산표관소화솔급장도조직형태적영향。선택7일령체중상근、건강적애발익가（ AA）육자계560지（공모각점1/2），수궤분위5개조，매조8개중복，매개중복14지계。대조조사위옥미-두박-육골분형기출사량，시험조분별사위재기출사량중첨가0．01％、0．02％、0．04％화0．08％단백매적시험사량，시험기14 d。결과표명：1）각단백매조육자계평균일채식량현저고우대조조（P＜0．05），0．02％단백매조육자계평균일증중현저고우대조조（ P＜0．05）。2）단백매가이차성제고육자계조단백질화안기산（량안산화이량안산제외）적표관소화솔（ P＜0．05），0．02％단백매조육자계조단백질표관소화솔현저고우대조조（ P＜0．05），0．01％화0．02％단백매조천동안산、소안산、사안산화병안산적표관소화솔균현저고우대조조（ P＜0．05）。3）수단백매첨가수평증가，십이지장은와심도정현저선성승고（P＜0．05），융모고도/은와심도정현저선성강저（P＜0．05）；공장융모고도정현저이차곡선승고（P＜0．05），은와심도정현저선성승고（P＜0．05）。유차가견，옥미-두박-육골분형사량첨가0．01％화0．02％단백매능구촉진육자계생장전기적소장발육，제고조단백질급대부분안기산표관소화솔，종이개선생장성능。
This experiment was conducted to study the effects of protease on growth performance, amino acid apparent digestibility and intestinal morphology of broilers aged from 8 to 21 days.A total of 560 seven-day-old healthy Arbor Acres broilers with a similar body weight were randomly allocated to 5 groups with 8 replicates per group and 14 chickens per replicate.Broilers in the control group were fed a basal diet, and broilers in the experimental groups were fed the basal diet supplemented with 0.01%, 0.02%, 0.04%and 0.08%protease, respectively.The experiment lasted for 14 days.The results showed as follows: 1) the average daily feed in-take of broilers in protease groups was significantly higher than that in control group ( P<0.05) , and the aver-age daily gain of broilers in 0.02%protease group was significantly higher than that in control group ( P<0.05) .2) The apparent digestibility of crude protein and amino acids ( except leucine and isoleucine) showed a quadratic curve of rising ( P<0.05) , the crude protein apparent digestibility of broilers in 0.02%protease group was significantly higher than that in control group ( P<0.05) , and the apparent digestibility of asparagic acid, threonine, serine and alanine in 0.01%and 0.02%protease groups was significantly higher than that in control group ( P<0.05) .3) With the increase of protease supplemental level, the crypt depth of duodenum showed a linear curve of rising ( P<0.05) , and the ratio of villus height to crypt depth ( V/C) showed a linear curve of falling ( P<0.05);the villus height of jejunum showed a quadratic curve of rising ( P<0.05) , and the crypt depth showed a linear curve of rising ( P<0.05) .It is concluded that adding 0.01%to 0.02%protease to the corn-soybean meal-meat and bone meal diet can promote the development of small intestine of broilers in the early stage, improve the apparent digestibility of crude protein and most of amino acids, and then improve the growth performance.