CAJ | 학술논문

目的：建立氧诱导视网膜新生血管小鼠模型，观察白蛋白在正常鼠和模型鼠视网膜中的含量变化，探讨白蛋白检测在评价血-视网膜屏障中的作用。方法取鼠龄7 d（P7）的健康C57BL/6J小鼠按随机数字表法分为模型组和正常对照组，模型组置于75％±2％氧气浓度的密闭氧舱中5 d，鼠龄12 d时（P12）返回正常空气环境以建立氧诱导鼠视网膜新生血管模型；正常对照组置于正常空气环境中饲养不予任何处理。模型组和正常对照组均在鼠龄17 d（P17）行FITC-Dextran左心室灌注视网膜铺片，荧光显微镜下观察视网膜血管分布和形态；组织切片HE 染色观察突破视网膜内界膜的血管内皮细胞。提取不同鼠龄（ P13、P15、P17）模型组和正常对照组视网膜总蛋白质，采用western blot技术检测视网膜组织中白蛋白的表达。结果荧光造影结果显示模型组视网膜有大量新生血管形成及明显的荧光素渗漏；组织切片显示模型组有大量突破视网膜内界膜的新生血管内皮细胞核；模型组视网膜白蛋白含量随缺氧时间延长而增加。结论在氧诱导鼠视网膜新生血管形成过程中白蛋白含量明显增加，其变化趋势与血-视网膜屏障破坏和视网膜新生血管形成具有时间上的一致性，白蛋白检测可以作为评价血-视网膜屏障有效指标。
목적：건립양유도시망막신생혈관소서모형，관찰백단백재정상서화모형서시망막중적함량변화，탐토백단백검측재평개혈-시망막병장중적작용。방법취서령7 d（P7）적건강C57BL/6J소서안수궤수자표법분위모형조화정상대조조，모형조치우75％±2％양기농도적밀폐양창중5 d，서령12 d시（P12）반회정상공기배경이건립양유도서시망막신생혈관모형；정상대조조치우정상공기배경중사양불여임하처리。모형조화정상대조조균재서령17 d（P17）행FITC-Dextran좌심실관주시망막포편，형광현미경하관찰시망막혈관분포화형태；조직절편HE 염색관찰돌파시망막내계막적혈관내피세포。제취불동서령（ P13、P15、P17）모형조화정상대조조시망막총단백질，채용western blot기술검측시망막조직중백단백적표체。결과형광조영결과현시모형조시망막유대량신생혈관형성급명현적형광소삼루；조직절편현시모형조유대량돌파시망막내계막적신생혈관내피세포핵；모형조시망막백단백함량수결양시간연장이증가。결론재양유도서시망막신생혈관형성과정중백단백함량명현증가，기변화추세여혈-시망막병장파배화시망막신생혈관형성구유시간상적일치성，백단백검측가이작위평개혈-시망막병장유효지표。
Objective To investigate expressions of albumin during retinal neovascularization in a murine model of oxygen -in-duced retinopathy.Methods C57BL/6J mice were exposed to (75 ±2)%oxygen for 5 days and returned to room air to induce retinal neovascularization .Retinal neovascularization was observed by fluorescence angiography , and was quantified by counting the endotheli-al nuclei protruding into the vitreous cavity after hematoxylin-eosin staining.The blood-retina barrier was evaluated by Western blot a-nalysis of extravasated albumin in the retina .Results In fluorescence angiograms , irregular neovascularization and fluorescein leakage was observed surrounding the unperfused areas in the hypoxic group .Compared to the normoxic group , there was a significant increase in both-side retinal albumin protein levels in the hypoxic group at P 13, P15 , and P17.Conclusions Albumin expression was in-creased in murine retina under hypoxia , and might be used to detect blood-retinal barrier damage of oxygen-induced retinopathy .