食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
7期
78-81
,共4页
分散液相微萃取%高效液相色谱法%婴儿奶瓶%双酚A
分散液相微萃取%高效液相色譜法%嬰兒奶瓶%雙酚A
분산액상미췌취%고효액상색보법%영인내병%쌍분A
DLLME%HPLC%baby bottle%Bisphenol A
建立了分散液相微萃取提取婴儿奶瓶中溶出痕量双酚A（BPA）的方法，并用高效液相色谱测定了其含量。奶瓶浸泡液用0.5 mL氯仿作萃取剂，0.5 mL甲醇作分散剂，以3500 r/min离心10 min，吸取萃取剂20μL，进样，采用高效液相色谱法（HPLC）测定BPA的含量。色谱柱为Inertsil C18柱（5μm，4.6×150 mm），流动相为甲醇∶水（V∶V）=70∶30，流速为0.8 mL/min，检测波长为280 nm。该方法BPA在0.05 mg/L~0.25 mg/L范围内线性关系良好（R=0.9997），BPA检出限（S/N=3）为0.50μg/L。该法用于婴儿奶瓶中BPA含量的检测，测得婴儿奶瓶中双酚A的含量范围在0~18.93μg/L，平均加标回收率为96.44%，RSD为4.57%（n=4）。方法结果满意，可适用于婴儿奶瓶、矿泉水等食品和饮料容器中溶出BPA的含量测定。
建立瞭分散液相微萃取提取嬰兒奶瓶中溶齣痕量雙酚A（BPA）的方法，併用高效液相色譜測定瞭其含量。奶瓶浸泡液用0.5 mL氯倣作萃取劑，0.5 mL甲醇作分散劑，以3500 r/min離心10 min，吸取萃取劑20μL，進樣，採用高效液相色譜法（HPLC）測定BPA的含量。色譜柱為Inertsil C18柱（5μm，4.6×150 mm），流動相為甲醇∶水（V∶V）=70∶30，流速為0.8 mL/min，檢測波長為280 nm。該方法BPA在0.05 mg/L~0.25 mg/L範圍內線性關繫良好（R=0.9997），BPA檢齣限（S/N=3）為0.50μg/L。該法用于嬰兒奶瓶中BPA含量的檢測，測得嬰兒奶瓶中雙酚A的含量範圍在0~18.93μg/L，平均加標迴收率為96.44%，RSD為4.57%（n=4）。方法結果滿意，可適用于嬰兒奶瓶、礦泉水等食品和飲料容器中溶齣BPA的含量測定。
건립료분산액상미췌취제취영인내병중용출흔량쌍분A（BPA）적방법，병용고효액상색보측정료기함량。내병침포액용0.5 mL록방작췌취제，0.5 mL갑순작분산제，이3500 r/min리심10 min，흡취췌취제20μL，진양，채용고효액상색보법（HPLC）측정BPA적함량。색보주위Inertsil C18주（5μm，4.6×150 mm），류동상위갑순∶수（V∶V）=70∶30，류속위0.8 mL/min，검측파장위280 nm。해방법BPA재0.05 mg/L~0.25 mg/L범위내선성관계량호（R=0.9997），BPA검출한（S/N=3）위0.50μg/L。해법용우영인내병중BPA함량적검측，측득영인내병중쌍분A적함량범위재0~18.93μg/L，평균가표회수솔위96.44%，RSD위4.57%（n=4）。방법결과만의，가괄용우영인내병、광천수등식품화음료용기중용출BPA적함량측정。
A novel method was developed for the determination of bisphenol A (BPA) in baby bottles sample by dispersive liquid-liquid microextraction (DLLME) coupled with high performance liquid chromatography (HPLC).In the method,0.50 mL chloromechane and 0.50 mL methanol were rapidly injected into a 5.0 mL sample water which bathing baby bottles.after centrifugation at 3 500 r/min for 10 min.the sedimented chloromechane phase was directly injected into the HPLC for analysis.The analysis was carried out on a Inertsil C18 column(5μm, 4.6×150 mm) with a mobile phase of methanol-H2O (V∶V=70∶30),at flow rate of 0.8 mL/min,the wave-length was 280 nm. Under the optimum conditions,BPA has a good linear relationship in the range of 0.05 mg/L-0.25 mg/L with the correlation coefficient of 0.999 7 and the detection limits of 0.50μg/L (S/N=3).This method has been successful applied for the determination of BPA in baby bottles ,The average contents of BPA in baby bottles was in the range of 0-18.93μg/L.The average relative recovery 96.44%,and the RSD was 4.57%(n=4), Samples were successfully analyzed by the proposed method with satisfactory results ,and the developed method was suitable for the detection of BPA in the food and beverage containers sample.
