CAJ | 학술논문

以豆渣为原料，黑曲霉、绿色木霉混合菌液为发酵菌种，制备高活性的水溶性大豆膳食纤维，并采用了响应面法对发酵工艺进行了优化。结果表明：实验范围内最佳工艺条件是黑曲霉∶绿色木霉=2∶1，发酵时间45 h，发酵温度26.4℃，菌种加液量10%，发酵液初pH6。得到的总膳食纤维（DF）得率为52.67%，水溶性膳食纤维（SDF）得率为22.76%，SDF约占DF的43.21%，大大的增加了豆渣中水溶性膳食纤维的含量。经冷冻干燥后，产品的感观性状为淡黄色，粉末颗粒度小，质地均匀，口感细腻，气味淡香。
이두사위원료，흑곡매、록색목매혼합균액위발효균충，제비고활성적수용성대두선식섬유，병채용료향응면법대발효공예진행료우화。결과표명：실험범위내최가공예조건시흑곡매∶록색목매=2∶1，발효시간45 h，발효온도26.4℃，균충가액량10%，발효액초pH6。득도적총선식섬유（DF）득솔위52.67%，수용성선식섬유（SDF）득솔위22.76%，SDF약점DF적43.21%，대대적증가료두사중수용성선식섬유적함량。경냉동간조후，산품적감관성상위담황색，분말과립도소，질지균균，구감세니，기미담향。
This paper focused on the preparation of high-activity soybean dietary fiber by fermentation with the soybean dregs as the material and the Asperillus niger and Trichoderma viride as the mixed bacteria, the optimization of the fermentation technology by Response Surface Methodology (RSM) was studied as well. The results showed that the optimum conditions of the fermentation technology were as follows: Asperillus niger:Trichoderma viride=2∶1(inoculum size), fermentation time 45 h, fermentation temperature 26.4℃, inoculation amount 10%and the initial pH value 6. Under the conditions, the yield of the soluble dietary fiber (SDF) was 22.76%, which accounted for 43.21%of the soluble dietary (DF). With the freeze-drying, the products has the characteristics of small particle size, uniform texture, exquisite taste, light yellow color and light aroma.