湖南农业科学
湖南農業科學
호남농업과학
HUNAN AGRICULTURAL SCIENCES
2014年
7期
62-65
,共4页
陈远超%朱春晖%孙书娥%刘勇%张德咏
陳遠超%硃春暉%孫書娥%劉勇%張德詠
진원초%주춘휘%손서아%류용%장덕영
小西葫芦黄花叶病毒(ZYMV)%RT-PCR%检测%湖南省
小西葫蘆黃花葉病毒(ZYMV)%RT-PCR%檢測%湖南省
소서호호황화협병독(ZYMV)%RT-PCR%검측%호남성
zucchini yellow mosaic virus%RT-PCR%detection%Hunan province
根据已报道的小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)保守序列设计了一对特异性引物,在58℃下退火30 s,建立了一种可靠、有效的ZYMV普通RT-PCR检测方法。通过该方法成功克隆出一条长度为621 bp的HC-Pro基因片段,并与杭州分离物的ZYMV辅助成分/蛋白酶(HC-Pro)基因核心区域(登录编号为AJ515911.1)的相似度达到99%。采用该方法对湖南省7个地区采集的疑似感病样品进行检测,结果显示:ZYMV平均阳性检出率达54.0%,说明ZYMV是侵染湖南省葫芦科作物的主要病毒之一。
根據已報道的小西葫蘆黃花葉病毒(Zucchini yellow mosaic virus,ZYMV)保守序列設計瞭一對特異性引物,在58℃下退火30 s,建立瞭一種可靠、有效的ZYMV普通RT-PCR檢測方法。通過該方法成功剋隆齣一條長度為621 bp的HC-Pro基因片段,併與杭州分離物的ZYMV輔助成分/蛋白酶(HC-Pro)基因覈心區域(登錄編號為AJ515911.1)的相似度達到99%。採用該方法對湖南省7箇地區採集的疑似感病樣品進行檢測,結果顯示:ZYMV平均暘性檢齣率達54.0%,說明ZYMV是侵染湖南省葫蘆科作物的主要病毒之一。
근거이보도적소서호호황화협병독(Zucchini yellow mosaic virus,ZYMV)보수서렬설계료일대특이성인물,재58℃하퇴화30 s,건립료일충가고、유효적ZYMV보통RT-PCR검측방법。통과해방법성공극륭출일조장도위621 bp적HC-Pro기인편단,병여항주분리물적ZYMV보조성분/단백매(HC-Pro)기인핵심구역(등록편호위AJ515911.1)적상사도체도99%。채용해방법대호남성7개지구채집적의사감병양품진행검측,결과현시:ZYMV평균양성검출솔체54.0%,설명ZYMV시침염호남성호호과작물적주요병독지일。
According to the reported conserved sequence of zucchini yellow mosaic virus (ZYMV), a pair of speciifc primer was designed and then annealed at 58℃ for 30 s, thereby successfully establishing an effective and reliable RT-PCR detection method for ZYMV. Through the method, a 621 bp ofHC-Pro gene segment was cloned successfully, and the similarity between it and the core gene region of ZYMV HC-Pro isolated from Hangzhou samples (the login ID is AJ515911.1) reached 99%. Using the method to detect suspected infected samples collected from seven areas of Hunan province, the results showed that the average positive detection rate of ZYMV was as high as 54.5%. Therefore, the ZYMV is one of the main viruses which infested in the cucurbitaceous crops in Hunan province.