CAJ | 학술논문

对8份不同产地的博落回花药进行了组织培养，通过对花药来源、4℃冷激时间、35℃热激时间、取蕾时期等因素的研究，建立了有效的博落回花药离体培养胚状体发生和植株再生体系。结果表明：有6个产地的博落回花药试材可以获得胚状体和再生植株；不同产地间出胚率差异显著，其中来自湖南高坊的材料出胚率最高，为11.8％；在4℃下冷激24~96 h有利于诱导出胚状体，冷激48 h的花药最容易产生胚状体；35℃热激处理对提高胚状体诱导率的作用不明显；花药培养的最佳取蕾时期为盛花期。
대8빈불동산지적박락회화약진행료조직배양，통과대화약래원、4℃랭격시간、35℃열격시간、취뢰시기등인소적연구，건립료유효적박락회화약리체배양배상체발생화식주재생체계。결과표명：유6개산지적박락회화약시재가이획득배상체화재생식주；불동산지간출배솔차이현저，기중래자호남고방적재료출배솔최고，위11.8％；재4℃하랭격24~96 h유리우유도출배상체，랭격48 h적화약최용역산생배상체；35℃열격처리대제고배상체유도솔적작용불명현；화약배양적최가취뢰시기위성화기。
A tissue culture was conducted to eight samples ofMacleaya cordata from different production areas, and the effective in vitro culture of anther and plant regeneration system forMacleaya cordata were established through studied the resources of anther, the cold-stress time (4℃), the heat-stress time (35℃), and the bud harvest time. The results showed that the anther samples ofMacleaya cordata from six production areas can obtain embryoid and regenerated plant; there was significant difference in embryo rate among different production areas, among which the sample from Gaofang of Hunan province had the highest embryo rate by 11.8%; cold-stress of anther at 4℃ for 24~96 h was beneficial for inducing embryoid, and the anther under cold-stress for 48 h was the most easy one to produce embryoid; the heat-stress treatment (35℃) had not signiifcant effect on improving inducing rate of embryoid; the optimal bud harvest time for anther culture was the full-bloom stage.