CAJ | 학술논문

微波辐射下经水提取得到小远志粗多糖（提取率4．86％），经过脱蛋白、二乙氨基乙基纤维素（DEAE-52）离子交换和Sephadex G-200凝胶色谱法分离得到两个主要组分PP-1和PP-2，纸层析、HPLC、比旋光度法证明两者均为均一组分，经凝胶层析法测得两者的相对分子质量分别为40800和97300．
미파복사하경수제취득도소원지조다당（제취솔4．86％），경과탈단백、이을안기을기섬유소（DEAE-52）리자교환화Sephadex G-200응효색보법분리득도량개주요조분PP-1화PP-2，지층석、HPLC、비선광도법증명량자균위균일조분，경응효층석법측득량자적상대분자질량분별위40800화97300．
Crud polysaccharide was isolated from Polygala sibirica L.var megalopha Fr.and extracted with water by microwave irradiation (yield 4. 86%).They were then purified through deproteinizated,DEAE-52 ion-exchange and Sephadex G-200 gel chromatography to get pure polysaccharide PP-1 and PP-2 .The results of the paper chro-matography,HPLC and specific rotation of PP-1 and PP-2 indicated that both of them were homogeneous compo-nent.The relative molecule weights of PP-1 and PP-2 were 40 800 and 97 300 respectively determined by gel filtra-tion chromatography.