CAJ | 학술논문

对湘西北、湘南、湘中和湘西等葡萄主产区的扇叶病发病及危害情况进行了调查，并通过生物学和分子生物学的方法对各地区的疑似病株进行了检测。结果表明：在湖南的葡萄主产区，葡萄扇叶病的感病品种较多，在夏黑无核、红地球、金星无核和金手指等品种上均有发生，但湘西的刺葡萄表现出对葡萄扇叶病有一定的抗性；感病症状总体上表现为叶片褪绿、黄化，叶缘锯齿变尖锐或形状不规则，并严重影响葡萄植株的生长，导致树势减弱、产量下降、果实品质变差；生物学检测结果显示，采集的54个样品，其中有52%的样品在千日红和本氏烟草上表现出系统性斑驳和变形；而分子生物学检测结果显示，54个样品中有29个样品检测到葡萄扇叶病病毒，以金星无核和红地球两个品种的检出率较高。
대상서북、상남、상중화상서등포도주산구적선협병발병급위해정황진행료조사，병통과생물학화분자생물학적방법대각지구적의사병주진행료검측。결과표명：재호남적포도주산구，포도선협병적감병품충교다，재하흑무핵、홍지구、금성무핵화금수지등품충상균유발생，단상서적자포도표현출대포도선협병유일정적항성；감병증상총체상표현위협편퇴록、황화，협연거치변첨예혹형상불규칙，병엄중영향포도식주적생장，도치수세감약、산량하강、과실품질변차；생물학검측결과현시，채집적54개양품，기중유52%적양품재천일홍화본씨연초상표현출계통성반박화변형；이분자생물학검측결과현시，54개양품중유29개양품검측도포도선협병병독，이금성무핵화홍지구량개품충적검출솔교고。
The occurrence situation and damages of grape fanleaf disease in main grape production areas in Hunan province, such as northwest Hunan, southern Hunan, middle Hunan and western Hunan, were investigated, and the suspected disease plants from each area were detected by biological method and molecular biological method. The results showed that in main grape production areas of Hunan province, many grape varieties occurred grape fanleaf disease, such as Xiahei-seedless, Red-earth, Jinxing-seedless, Golden-finger, etc., but the Vitis davidii var. davidii from western Hunan showed a certain resistance to the grape fanleaf disease. The symptoms of the grape fanleaf disease generally showed as follows: the leaf was degreening and yellowing; the serrate leaf margin turned to sharpening or out-of-shape; the growth of grape plants was influenced seriously, thus weakening tree vigour, reducing yield and decreasing fruit quality. The biological detection results indicated that among 54 collected samples, 52% samples presented systemic mottle and distortion in Qianrihong and Benshi tobacco varieties. Moreover, the molecular biological detection results revealed that 29 out of 54 samples detected grape fanleaf disease virus, and the detection rate of the Jinxing-seedless and the Red-earth was relatively high.