CAJ | 학술논문

目的：评价改良静态细胞种植方法在培育二维心血管组织工程补片中的价值。方法将人脐带动脉壁平滑肌细胞以静态细胞种植方法种植在聚-4-羟基丁酸酯材料构建的补片支架材料上，制备组织工程补片。分别采用两种方法进行培育：静态培育为细胞材料复合物体外静态培育21 d；动态培育为细胞材料复合物先体外静态培育14 d，再置入生物反应器中继续动态组织培育7 d。对补片组织进行组织学、扫描电镜检查。免疫组化分析细胞外基质合成。结果静态细胞种植后经体外动态培育后，平滑肌细胞均匀地黏附于材料表面，细胞保持生长活力，并浸润生长入材料内部，形成均匀组织。结论改良静态细胞种植方法在二维组织工程支架材料上可达到细胞及组织的均匀分布。
목적：평개개량정태세포충식방법재배육이유심혈관조직공정보편중적개치。방법장인제대동맥벽평활기세포이정태세포충식방법충식재취-4-간기정산지재료구건적보편지가재료상，제비조직공정보편。분별채용량충방법진행배육：정태배육위세포재료복합물체외정태배육21 d；동태배육위세포재료복합물선체외정태배육14 d，재치입생물반응기중계속동태조직배육7 d。대보편조직진행조직학、소묘전경검사。면역조화분석세포외기질합성。결과정태세포충식후경체외동태배육후，평활기세포균균지점부우재료표면，세포보지생장활력，병침윤생장입재료내부，형성균균조직。결론개량정태세포충식방법재이유조직공정지가재료상가체도세포급조직적균균분포。
Objective To evaluate the application of modified static cell seeding method in tissue engineering of 2-dimensional cardiovascular patch .Methods Patch scaffolds fabricated from poly -4 -hydroxy -butyrate ( P4HB) polymers were seeded with human umbilical artery myofibroblasts ( HUAMs) using static cell seeding method .In static cultivation group , the cell-polymer patch constructs were cultured in vitro for 21 days under static cell culture condition . In dynamic group , the cell-seeded patch constructs were incubated in vitro for 14 days under static culture conditions and were subsequently transferred into a bioreactor for another culture of 7 days.Morphological characterization of tissue engi-neered ( TE) patches was observed included histology and scanning electron microscopy ( SEM ) .Extracellular matrix ( collagen ) formation was analyzed by immunohistochemistry .Results After static cell seeding and subsequently condi-tioning in the bioreactor , the fibroblast cells were well adhered to the surface of P 4HB polymer, the cells were mostly via-ble, had grown into the pores , and had formed uniform tissue on the patch constructs .Conclusion Using modified static cell seeding method , the uniform distribution of cells and tissue on the 2-dimensional tissue engineered structures can be achieved.