CAJ | 학술논문

目的：探讨miR-195对胃癌细胞增殖、迁移及侵袭的影响。方法以人胃癌细胞系SGC-7901细胞株及BGC-823细胞株为研究对象，分别转染miR-195模拟物及抑制物。应用实时荧光定量PCR技术，检测转染后miR-195 mRNA的表达水平。通过CCK8方法检测miR-195对胃癌细胞增殖的影响。通过Transwell实验检测miR-195对胃癌细胞迁移和侵袭的影响。结果（1） CCK8实验研究显示，与阴性对照（无关序列）相比，转染miR-195模拟物后，SGC-7901细胞的增殖能力减弱；转染miR-195抑制物后，BGC-823细胞的增殖能力明显增强。（2） Transwell小室实验研究显示，与阴性对照相比，转染miR-195模拟物后，SGC-7901细胞的迁移及侵袭能力明显下降；转染miR-195抑制物后，BGC-823细胞的迁移及侵袭能力明显增强。结论 miR-195在胃癌细胞的增殖、迁移及侵袭中发挥重要的作用，miR-195可能成为治疗胃癌的靶基因。
목적：탐토miR-195대위암세포증식、천이급침습적영향。방법이인위암세포계SGC-7901세포주급BGC-823세포주위연구대상，분별전염miR-195모의물급억제물。응용실시형광정량PCR기술，검측전염후miR-195 mRNA적표체수평。통과CCK8방법검측miR-195대위암세포증식적영향。통과Transwell실험검측miR-195대위암세포천이화침습적영향。결과（1） CCK8실험연구현시，여음성대조（무관서렬）상비，전염miR-195모의물후，SGC-7901세포적증식능력감약；전염miR-195억제물후，BGC-823세포적증식능력명현증강。（2） Transwell소실실험연구현시，여음성대조상비，전염miR-195모의물후，SGC-7901세포적천이급침습능력명현하강；전염miR-195억제물후，BGC-823세포적천이급침습능력명현증강。결론 miR-195재위암세포적증식、천이급침습중발휘중요적작용，miR-195가능성위치료위암적파기인。
Objective To investigate the effect of microRNA -195 (miR-195) on the proliferation, migration and invasion of gastric cancer cells .Methods Human gastric cancer cell lines , including SGC-7901 cells and BGC-823 cells, were selected, and were transfected with miR -195 mimics and inhibitors, respectively.The expression level of miR-195 mRNA was assessed using real time fluorescent quantitative PCR after transfection .Through the CCK8 method and transwell assay , the effect of miR-195 on migration and invasion of gastric cancer cells was analyzed .Results Ac-cording to CCK8 test, significant inhibition on the proliferation of SGC -7901 cells was produced by transfection of miR -195 mimics compared with the negative control group ( unrelated sequence );while significant enhancement in proliferation of BGC-823 cells was observed .According to transwell assay , compared with negative control group , transfection of miR-195 mimics significantly inhibited the migration and invasion of SGC -7901 cells;while transfection of miR-195 inhibitor enhanced them .Conclusion miR-195 play an important role on proliferation , migration and invasion of gastric cancer cells, suggesting a potential targeted gene in therapy of gastric cancer .