中国保健营养（中旬刊）
中國保健營養（中旬刊）
중국보건영양（중순간）
China Hwalth Care & nutrition
2013年
5期
57-59
,共3页
OPG%基因敲除%椎间盘退变
OPG%基因敲除%椎間盤退變
OPG%기인고제%추간반퇴변
OPG%gene knock-out%intervertebral disc degeneration
目的：比较骨保护素（OPG）基因敲除（OPG-/-）小鼠与正常（WT）小鼠腰椎间盘退变的关系。方法：分别取出生后4W、8W、12W的OPG-/-小鼠及正常对照组小鼠的L4/5椎间盘，运用番红O-固绿染色法观察L4/5椎间盘形态学变化，测量椎间盘及软骨终板高度。应用免疫组化染色观察椎间盘聚集蛋白聚糖(aggrecan)基因表达量的变化。结果：1.小鼠腰椎椎间盘番红O-固绿染色结果：OPG-/-小鼠的椎间盘软骨终板在第8W及第12W 时可观察到退化改变：软骨终板排列不规则，并有骨髓腔组织进入软骨终板及外层纤维环。2.免疫组化染色结果显示：各年龄点 OPG-/-组小鼠椎间盘 aggrecan 的蛋白表达均低于同龄WT 组小鼠。3. OPG-/-小鼠软骨终板及椎间盘高度在4周时较正常对照组无明显变化，随着年龄的增长，第8周时较对照组降低，第12周时高度下降最明显(P<0.01)。讨论：OPG基因缺失后可导致椎间盘退变，椎间盘正常的结构和功能的维持有赖于OPG基因。
目的：比較骨保護素（OPG）基因敲除（OPG-/-）小鼠與正常（WT）小鼠腰椎間盤退變的關繫。方法：分彆取齣生後4W、8W、12W的OPG-/-小鼠及正常對照組小鼠的L4/5椎間盤，運用番紅O-固綠染色法觀察L4/5椎間盤形態學變化，測量椎間盤及軟骨終闆高度。應用免疫組化染色觀察椎間盤聚集蛋白聚糖(aggrecan)基因錶達量的變化。結果：1.小鼠腰椎椎間盤番紅O-固綠染色結果：OPG-/-小鼠的椎間盤軟骨終闆在第8W及第12W 時可觀察到退化改變：軟骨終闆排列不規則，併有骨髓腔組織進入軟骨終闆及外層纖維環。2.免疫組化染色結果顯示：各年齡點 OPG-/-組小鼠椎間盤 aggrecan 的蛋白錶達均低于同齡WT 組小鼠。3. OPG-/-小鼠軟骨終闆及椎間盤高度在4週時較正常對照組無明顯變化，隨著年齡的增長，第8週時較對照組降低，第12週時高度下降最明顯(P<0.01)。討論：OPG基因缺失後可導緻椎間盤退變，椎間盤正常的結構和功能的維持有賴于OPG基因。
목적：비교골보호소（OPG）기인고제（OPG-/-）소서여정상（WT）소서요추간반퇴변적관계。방법：분별취출생후4W、8W、12W적OPG-/-소서급정상대조조소서적L4/5추간반，운용번홍O-고록염색법관찰L4/5추간반형태학변화，측량추간반급연골종판고도。응용면역조화염색관찰추간반취집단백취당(aggrecan)기인표체량적변화。결과：1.소서요추추간반번홍O-고록염색결과：OPG-/-소서적추간반연골종판재제8W급제12W 시가관찰도퇴화개변：연골종판배렬불규칙，병유골수강조직진입연골종판급외층섬유배。2.면역조화염색결과현시：각년령점 OPG-/-조소서추간반 aggrecan 적단백표체균저우동령WT 조소서。3. OPG-/-소서연골종판급추간반고도재4주시교정상대조조무명현변화，수착년령적증장，제8주시교대조조강저，제12주시고도하강최명현(P<0.01)。토론：OPG기인결실후가도치추간반퇴변，추간반정상적결구화공능적유지유뢰우OPG기인。
Objective:To observe the relationship between the degeneration of the lumbar intervertebral disc (IVD) in OPG gene knock-out mice and WT mice.Menthods:The L4/5 lumbar intervertebral discs (L4/5 IVD) from 4-, 8-, 12-week-old mice were harvested. Routine morphologic observations of the intervertebral discs and cartilage endplate were performed using safranin-O/fast Green staining. The height of the intervertebral discs and the thickness of cartilage endplate were measured;Protein expressions of aggrecan in the discs were detected with immunohistochemical analysis.Results:Safranin-O/fast green staining exhibited degeneration of the intervertebral discs and cartilage endplates in OPG-/-mice characterized with thinning, disordered arrangement of cartilage endplates and appearance of bone marrow cavity both in the endplate cartilage and annulus fibrosis. Immunohistochemical assay revealed significantly less protein expressions of aggrecan in the discs from OPG-/-mice than from wild type mice. Compared with the control group OPG-/- mice cartilage endplate and intervertebral disc height have no significant change at 4W, Increase with age, lower than control group at 8W,height decreased significantly at 12W.(P<0.01).Conclusion:OPG gene plays a very important role in maintaining the structure and function of normal intervertebral disc, the deletion of OPG gene causes intervertebral disc degeration and vertebra osteoporosis.
