中国保健营养(中旬刊)
中國保健營養(中旬刊)
중국보건영양(중순간)
China Hwalth Care & nutrition
2013年
7期
195-196
,共2页
杨冬燕%杨永存%李浩%耿艺介%张倩%吴双%邓平建
楊鼕燕%楊永存%李浩%耿藝介%張倩%吳雙%鄧平建
양동연%양영존%리호%경예개%장천%오쌍%산평건
地沟油%同源异形盒家族%HOXC6基因%荧光PCR
地溝油%同源異形盒傢族%HOXC6基因%熒光PCR
지구유%동원이형합가족%HOXC6기인%형광PCR
Trench oil%homeobox family%HOXC6 gene%real-time PCR
目的:以动物源性的同源异形盒家族 HOXC6基因为靶标建立地沟油鉴定方法。方法:通过基因数据库的筛查、比对及特异性验证,筛选确定以动物源性的同源异形盒家族HOXC6基因为靶标,采用实时荧光PCR方法对正常植物油和地沟油进行鉴定。结果:6个已知来源的地沟油样品全部被鉴定为地沟油,而8个正常食用植物油全部被鉴定为非地沟油。结论:与课题组前期建立的采用针对多个动物物种特异性基因的PCR鉴定方法相比,该研究建立的方法在有效提高检测灵敏度的同时,不但避免了先后扩增多个基因片段的繁琐,同时降低了PCR污染的风险,提高了检测结果的准确性。
目的:以動物源性的同源異形盒傢族 HOXC6基因為靶標建立地溝油鑒定方法。方法:通過基因數據庫的篩查、比對及特異性驗證,篩選確定以動物源性的同源異形盒傢族HOXC6基因為靶標,採用實時熒光PCR方法對正常植物油和地溝油進行鑒定。結果:6箇已知來源的地溝油樣品全部被鑒定為地溝油,而8箇正常食用植物油全部被鑒定為非地溝油。結論:與課題組前期建立的採用針對多箇動物物種特異性基因的PCR鑒定方法相比,該研究建立的方法在有效提高檢測靈敏度的同時,不但避免瞭先後擴增多箇基因片段的繁瑣,同時降低瞭PCR汙染的風險,提高瞭檢測結果的準確性。
목적:이동물원성적동원이형합가족 HOXC6기인위파표건입지구유감정방법。방법:통과기인수거고적사사、비대급특이성험증,사선학정이동물원성적동원이형합가족HOXC6기인위파표,채용실시형광PCR방법대정상식물유화지구유진행감정。결과:6개이지래원적지구유양품전부피감정위지구유,이8개정상식용식물유전부피감정위비지구유。결론:여과제조전기건립적채용침대다개동물물충특이성기인적PCR감정방법상비,해연구건립적방법재유효제고검측령민도적동시,불단피면료선후확증다개기인편단적번쇄,동시강저료PCR오염적풍험,제고료검측결과적준학성。
Objective:To develop a specific identification method for trench oil by HOXC6 gene amplification. Methods:Though the steps of genebank screening, comparison and specific verification, the HOXC6 gene in homeobox family is determined as the target to identify the trench oil from normal vegetable oil using real-time PCR. Results:6 trench oil samples were accurately identified from 8 edible vegetable oil samples. Conclusion:Compared with the former research established by our group, the novel method effectively improves the detection sensitivity and accuracy. At the same time, it not only avoids the complication from multiple amplification, but also reduces the risk of PCR contamination.