中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
6期
1331-1333
,共3页
大肠癌%促血管生成素-2
大腸癌%促血管生成素-2
대장암%촉혈관생성소-2
Colon carcinomas%Angiopoietin-2
目的 观察促血管生成素-2(Ang-2)在大肠癌组织中的表达及其与临床病理的联系,并探讨其临床意义.方法 采用逆转录-聚合酶链反应(RT-PCR)法检测40例大肠癌组织和正常肠黏膜中Ang-2 mRNA的表达,另用Western blot法检测其中10例肿瘤组织和正常组织中Ang-2蛋白的表达;用免疫组织化学法检测其中10例肿瘤组织中Ang-2蛋白的表达.结果 60.0％ (24/40)的大肠癌组织中Ang-2 mRNA强表达,而正常肠黏膜中7.5％ (3/40)弱表达,36例阳性表达的肿瘤组织中Ang-2 mRNA表达的平均吸光度值为4.329 6±0.280 9,而30例正常黏膜上皮的平均吸光度值为3.309 7±0.291 4,两者差异有统计学意义(P＜0.01);Ang-2蛋白在大肠癌中的表达与肿瘤的病理类型、侵犯层次、淋巴结转移和病理分期有关,且随病程进展表达呈增强趋势.结论 Ang-2参与了大肠癌组织新生血管的形成及维持,与大肠癌的进程一致.
目的 觀察促血管生成素-2(Ang-2)在大腸癌組織中的錶達及其與臨床病理的聯繫,併探討其臨床意義.方法 採用逆轉錄-聚閤酶鏈反應(RT-PCR)法檢測40例大腸癌組織和正常腸黏膜中Ang-2 mRNA的錶達,另用Western blot法檢測其中10例腫瘤組織和正常組織中Ang-2蛋白的錶達;用免疫組織化學法檢測其中10例腫瘤組織中Ang-2蛋白的錶達.結果 60.0％ (24/40)的大腸癌組織中Ang-2 mRNA彊錶達,而正常腸黏膜中7.5％ (3/40)弱錶達,36例暘性錶達的腫瘤組織中Ang-2 mRNA錶達的平均吸光度值為4.329 6±0.280 9,而30例正常黏膜上皮的平均吸光度值為3.309 7±0.291 4,兩者差異有統計學意義(P＜0.01);Ang-2蛋白在大腸癌中的錶達與腫瘤的病理類型、侵犯層次、淋巴結轉移和病理分期有關,且隨病程進展錶達呈增彊趨勢.結論 Ang-2參與瞭大腸癌組織新生血管的形成及維持,與大腸癌的進程一緻.
목적 관찰촉혈관생성소-2(Ang-2)재대장암조직중적표체급기여림상병리적련계,병탐토기림상의의.방법 채용역전록-취합매련반응(RT-PCR)법검측40례대장암조직화정상장점막중Ang-2 mRNA적표체,령용Western blot법검측기중10례종류조직화정상조직중Ang-2단백적표체;용면역조직화학법검측기중10례종류조직중Ang-2단백적표체.결과 60.0％ (24/40)적대장암조직중Ang-2 mRNA강표체,이정상장점막중7.5％ (3/40)약표체,36례양성표체적종류조직중Ang-2 mRNA표체적평균흡광도치위4.329 6±0.280 9,이30례정상점막상피적평균흡광도치위3.309 7±0.291 4,량자차이유통계학의의(P＜0.01);Ang-2단백재대장암중적표체여종류적병리류형、침범층차、림파결전이화병리분기유관,차수병정진전표체정증강추세.결론 Ang-2삼여료대장암조직신생혈관적형성급유지,여대장암적진정일치.
Objective To evaluate the expression of angiopoietin-2 (Ang-2) in human colon carcinomas tissues and its relation with clinicopathological parameters in order to clarify the role of Ang-2 in the progression of tumors.Methods Forty cases of colon carcinomas were studied.The relationship between the mRNA expression of Ang-2 and clinicopathological features were examined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.Immunohistochemical method was used to detect the expression of Ang-2 protein in 10 samples of cancer tissue.Results Ang-2 mRNA expression was significantly detected in 60.0％ of human colon carcinomas tissues and 7.5％ of normal tissues,the expression of Ang-2 mRNA in 36 cases of positive expression in tumor tissue,the average absorbance value was 4.329 6 ± 0.280 9,while the average absorbance of 30 normal mucosal epithelial values for 3.309 7 ± 0.291 4 (P ＜0.01).The Ang-2 expression was related to the pathological types,nodal invasion and lymph node metastasis stage of colon carcinomas.The patients with colon carcinoma strongly positive for Ang-2-expression showed more advanced stages or disease than those weakly positive for Ang-2 expression.Conclusion Ang-2 might be an important factor in tumor angiogenesis and growth in human colon carcinomas.
