中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
12期
2695-2697
,共3页
微小RNA-650%肝细胞癌%生长抑制因子
微小RNA-650%肝細胞癌%生長抑製因子
미소RNA-650%간세포암%생장억제인자
MicroRNA-650%Hepatocellular cancer%Inhibitor of growth family member
目的 探讨微小RNA (miR)-650与肝细胞癌(HCC)发生发展的关系.方法 实时定量聚合酶链反应(Real-time PCR)检测并比较肝癌及其癌旁组织、正常肝组织中miR-650的表达水平,分析其与临床病理特征的关系;正常肝细胞株THLE-2分别转染miR-650双链体、miR-650反义序列,Real-time PCR、Western blot、噻唑蓝(MTT)分别用于检测转染效率、生长抑制因子-4(ING4)蛋白表达及细胞增殖活力.结果 miR-650的表达水平在HCC组织中高于癌旁组织(3.02±1.11比1.25±0.63,P＜0.05),并与患者的年龄(P＜0.01)、肿瘤分化程度(p＜0.05)和肿瘤分期(P＜0.01)明显相关.miR-650与ING4的表达明显负相关(rs=-0.2011,P＜0.05).肝细胞增殖活力在转染后72、96、120、144 h较对照组明显活跃(1.53 ±0.15比0.88±0.11,2.62 ±0.26比1.44±0.18,3.82±0.29比2.53±0.24,4.06±0.24比3.06±0.35,p＜0.05).结论 miR-650与HCC的临床病理学特征有关,它可能通过抑制ING4的表达而促进了HCC的肿瘤发生发展.
目的 探討微小RNA (miR)-650與肝細胞癌(HCC)髮生髮展的關繫.方法 實時定量聚閤酶鏈反應(Real-time PCR)檢測併比較肝癌及其癌徬組織、正常肝組織中miR-650的錶達水平,分析其與臨床病理特徵的關繫;正常肝細胞株THLE-2分彆轉染miR-650雙鏈體、miR-650反義序列,Real-time PCR、Western blot、噻唑藍(MTT)分彆用于檢測轉染效率、生長抑製因子-4(ING4)蛋白錶達及細胞增殖活力.結果 miR-650的錶達水平在HCC組織中高于癌徬組織(3.02±1.11比1.25±0.63,P＜0.05),併與患者的年齡(P＜0.01)、腫瘤分化程度(p＜0.05)和腫瘤分期(P＜0.01)明顯相關.miR-650與ING4的錶達明顯負相關(rs=-0.2011,P＜0.05).肝細胞增殖活力在轉染後72、96、120、144 h較對照組明顯活躍(1.53 ±0.15比0.88±0.11,2.62 ±0.26比1.44±0.18,3.82±0.29比2.53±0.24,4.06±0.24比3.06±0.35,p＜0.05).結論 miR-650與HCC的臨床病理學特徵有關,它可能通過抑製ING4的錶達而促進瞭HCC的腫瘤髮生髮展.
목적 탐토미소RNA (miR)-650여간세포암(HCC)발생발전적관계.방법 실시정량취합매련반응(Real-time PCR)검측병비교간암급기암방조직、정상간조직중miR-650적표체수평,분석기여림상병리특정적관계;정상간세포주THLE-2분별전염miR-650쌍련체、miR-650반의서렬,Real-time PCR、Western blot、새서람(MTT)분별용우검측전염효솔、생장억제인자-4(ING4)단백표체급세포증식활력.결과 miR-650적표체수평재HCC조직중고우암방조직(3.02±1.11비1.25±0.63,P＜0.05),병여환자적년령(P＜0.01)、종류분화정도(p＜0.05)화종류분기(P＜0.01)명현상관.miR-650여ING4적표체명현부상관(rs=-0.2011,P＜0.05).간세포증식활력재전염후72、96、120、144 h교대조조명현활약(1.53 ±0.15비0.88±0.11,2.62 ±0.26비1.44±0.18,3.82±0.29비2.53±0.24,4.06±0.24비3.06±0.35,p＜0.05).결론 miR-650여HCC적림상병이학특정유관,타가능통과억제ING4적표체이촉진료HCC적종류발생발전.
Objective The aim of this article is to explore the relationship between microRNA (miR)-650 and hepatocellular cancer (HCC).Methods The expression level of miR-650 in HCC tissues,paracarcinomatous liver(PCL) tissues and normal liver tissues was examined by real-time quantitative polymerase chain reaction (Real-time PCR).Western blotting was performed to examine miR-650 expression level and inhibitor of growth family member 4 (ING4) protein.Normal liver cells THLE-2 were transfected with miR-650 duplex and miR-650 single antisence sequence.Real-time PCR was used to investigate miRNA transfection efficiencv.Western blotting was used to detect differences of ING4 protein expression.Cell viability was measured using the methyl thiazol tetrazolium (MTT) assay.Results The expression level of miR-650 was significantly increased in HCC tissues compared to PCL tissues (3.02 ± 1.11 vs 1.25 ± 0.63,P ＜ 0.05).and obviously correlated with the patient' s age (P ＜ 0.01),tumor stage (P ＜ 0.01) and differentiation capability (P ＜ 0.01).There existed a significant negative association between miR-650 and ING4 (rs =-0.2011,P ＜ 0.05).Transfection suggested that miR-650 decreased the expression of ING4 and stimulated cell viability at 72 h,96 h,120 h,144 h (1.53 ± 0.1vs0.88 ± 0.11,2.62 ±0.2 vs 1.44±0.18,3.82 ±.0.2 vs 2.53 ±0.24,4.06±0.24 vs 3.06 ±0.35,all P＜0.05).Conclusion The statistical analysis data showed that miR-650 was correlated with the clinicopathological characteristics of HCC,it may be involved in the tumorigenesis of HCC by inhibiting the expression of ING4.
