CAJ | 학술논문

为研究产乙醇基因工程集胞藻培养液中的乙醇消耗菌污染情况及其对乙醇产量的影响，从污染的培养液中分离出4株乙醇消耗菌，通过16S rDNA、26S rDNA序列分析对分离出的菌株进行鉴定，并研究其乙醇消耗能力及对基因工程集胞藻乙醇产量的影响。结果表明，分离出的4株菌分别为红酵母(Rhodotorula sp.)、季也蒙酵母(Meyerozyma guilliermondii)、短波单胞菌(Brevundimonas sp.)、微杆菌(Microbacterium sp.)。其中乙醇消耗能力最强的是红酵母，乙醇比消耗速率达到391 g/(1015 cfu?d)；其次为季也蒙酵母，乙醇比消耗速率为80.1 g/(1015 cfu?d)；短波单胞菌和微杆菌的乙醇比消耗速率远低于红酵母和季也蒙酵母。将分离出的菌株与产乙醇集胞藻共培养7d 后，污染红酵母、季也蒙酵母、短波单胞菌、微杆菌的实验组乙醇产量分别下降了53.8%、23.6%、40.7%、27.3%。4株菌对基因工程集胞藻的生长无明显影响，均通过直接消耗乙醇而降低集胞藻的乙醇产量。
위연구산을순기인공정집포조배양액중적을순소모균오염정황급기대을순산량적영향，종오염적배양액중분리출4주을순소모균，통과16S rDNA、26S rDNA서렬분석대분리출적균주진행감정，병연구기을순소모능력급대기인공정집포조을순산량적영향。결과표명，분리출적4주균분별위홍효모(Rhodotorula sp.)、계야몽효모(Meyerozyma guilliermondii)、단파단포균(Brevundimonas sp.)、미간균(Microbacterium sp.)。기중을순소모능력최강적시홍효모，을순비소모속솔체도391 g/(1015 cfu?d)；기차위계야몽효모，을순비소모속솔위80.1 g/(1015 cfu?d)；단파단포균화미간균적을순비소모속솔원저우홍효모화계야몽효모。장분리출적균주여산을순집포조공배양7d 후，오염홍효모、계야몽효모、단파단포균、미간균적실험조을순산량분별하강료53.8%、23.6%、40.7%、27.3%。4주균대기인공정집포조적생장무명현영향，균통과직접소모을순이강저집포조적을순산량。
Four ethanol consuming microorganisms (GR13, GW13, GT17, and GY22) were isolated from the contami-nated culture of genetically engineered Synechocystis sp. and were utilized to evaluate the effects of these microorga-nisms on the ethanol consuming capabilities and ethanol productions of Synechocystis sp. Based on the morphological characteristics and 16S rDNA and 26S rDNA gene sequence, the four strains were identified as Rhodotorula sp., Meye-rozyma sp., Brevundimonas sp., and Microbacterium sp., respectively. We observed that strain GR13 (Rhodotorula sp.) had the highest level of specific ethanol consumption rate [391 g/(1015 cfu?d)], followed by strain GW13 (Meyerozyma sp., [ 80.1 g/(1015 cfu?d)]. While strains GT17 (Brevundimonas sp.) and GY22 (Microbacterium sp.) had much lower ethanol consumption rate. All four strains did not significantly affect the growth of Synechocystis sp., however, they remarkably decreased the ethanol production of Synechocystis sp. The results of the co-culture experiment demonstrated that strains GR13, GW13, GT17, and GY22 diminished ethanol production of the genetically engineered Synechocystis sp. by 53.8%, 23.6%, 40.7%and 27.3%, respectively.