CAJ | 학술논문

系统研究了小球藻FACHB 484在含有葡萄糖的不同营养方式下的生长情况，并通过抑制试验探讨葡萄糖在小球藻FACHB 484光异养和兼养生长条件下所起的作用以及小球藻FACHB 484是否存在氧化呼吸系统的关键酶类。结果表明：小球藻FACHB 484可利用葡萄糖进行化能异养、光激活异养、光异养及兼养生长，其生长速率大小为：兼养＞光异养＞光激活异养＞化能异养＞光合自养。兼养培养的最大生物量和比生长速率分别是自养培养的8.6和3.4倍，其比生长速率接近于光合自养和光异养培养下的比生长速率之和。葡萄糖主要作为小球藻FACHB 484兼养和光异养培养的碳源，而能量主要源自光。小球藻FACHB 484存在氧化呼吸链代谢途径，其细胞中有琥珀酸脱氢酶和细胞色素氧化酶。
계통연구료소구조FACHB 484재함유포도당적불동영양방식하적생장정황，병통과억제시험탐토포도당재소구조FACHB 484광이양화겸양생장조건하소기적작용이급소구조FACHB 484시부존재양화호흡계통적관건매류。결과표명：소구조FACHB 484가이용포도당진행화능이양、광격활이양、광이양급겸양생장，기생장속솔대소위：겸양＞광이양＞광격활이양＞화능이양＞광합자양。겸양배양적최대생물량화비생장속솔분별시자양배양적8.6화3.4배，기비생장속솔접근우광합자양화광이양배양하적비생장속솔지화。포도당주요작위소구조FACHB 484겸양화광이양배양적탄원，이능량주요원자광。소구조FACHB 484존재양화호흡련대사도경，기세포중유호박산탈경매화세포색소양화매。
Here we systematically studied the growth of Chlorella sp. FACHB 484 in glucose-containing medium in different nutrition modes. We also conducted the inhibition tests to investigate the effects of glucose on the growth of Chlorella sp. FACHB 484 in heterotrophic and mixotrophic conditions, as well as to identify the key enzyme in oxida-tion respiratory system. We observed that Chlorella sp. FACHB 484 could utilize glucose as the source of nutrition in all the test nutritional modes and showed different growth rates in the order mixotrophy > photoheterotrophy >light-activated heterotrophy > chemoheterotrophy > photoautotrophy. The maximum biomass and specific growth rate (SGR) of the tested algae in mixotrophy were 8.6 and 3.4 times higher than those in photoautotrophy. The value of SGR of Chlorella sp. FACHB 484 in mixotrophy was close to the sum of the values of SGRs in photoautotrophy and photo-heterotrophy. In mixotrophic and photoheterotrophic modes, Chlorella sp. FACHB 484 used glucose and light as the sources of carbon and energy respectively. The cells of Chlorella sp. FACHB 484 expresses succinic dehydrogenase and cytochrome oxidase which are most likely the key enzymes of the oxidation respiratory chain.