CAJ | 학술논문

构建了建鲤(Cyprinus carpio var. jian)鸟氨酸脱羧酶(Ornithine decarboxylase, ODC) jlODC1a基因上6个和jlODC1b基因上4个SNP位点的PCR-RFLP方法,检测了这10个位点在12个家系约900尾建鲤选育群体中的基因型,各位点的基因型频率存在差异,最小等位基因频率(MAF)为0.14-0.48。各位点不同基因型与增重相关分析结果,其中7个 SNPs 与建鲤增重显性相关的位点, ODC1s 基因上与雌鱼增重相关的SNP位点(7个)较雄鱼(4个)多。标记富集结果表明富集与建鲤增重相关的优势基因型的SNP个数越多的个体增重速度越快 SNP 个数越多的个体增重速度越快,富集4个的平均增重显著快于富集0-3的个体增重,且比0标记的快约14%,这反映出生长为数量性状。进一步对所检测位点进行双倍型分析,结果显示具有四个优势基因型且全部杂合优势基因型的4567(XXXXXXACCTCTCT)组的增重最快,比0优势基因型的增重快达26.6%,可以考虑用于今后的快增长建鲤的选育计划中。此外,双倍型分析结果还表明,不同位点之间可能存在或颉抗或协同的互作,如1和4之间存在拮抗关系,因此在今后的选育计划中,在考虑标记富集的情况下还应考虑标记之间的关系。宜在选择互为协同作用优势基因型的前提下,富集尽可能多的SNP标记。
구건료건리(Cyprinus carpio var. jian)조안산탈최매(Ornithine decarboxylase, ODC) jlODC1a기인상6개화jlODC1b기인상4개SNP위점적PCR-RFLP방법,검측료저10개위점재12개가계약900미건리선육군체중적기인형,각위점적기인형빈솔존재차이,최소등위기인빈솔(MAF)위0.14-0.48。각위점불동기인형여증중상관분석결과,기중7개 SNPs 여건리증중현성상관적위점, ODC1s 기인상여자어증중상관적SNP위점(7개)교웅어(4개)다。표기부집결과표명부집여건리증중상관적우세기인형적SNP개수월다적개체증중속도월쾌 SNP 개수월다적개체증중속도월쾌,부집4개적평균증중현저쾌우부집0-3적개체증중,차비0표기적쾌약14%,저반영출생장위수량성상。진일보대소검측위점진행쌍배형분석,결과현시구유사개우세기인형차전부잡합우세기인형적4567(XXXXXXACCTCTCT)조적증중최쾌,비0우세기인형적증중쾌체26.6%,가이고필용우금후적쾌증장건리적선육계화중。차외,쌍배형분석결과환표명,불동위점지간가능존재혹힐항혹협동적호작,여1화4지간존재길항관계,인차재금후적선육계화중,재고필표기부집적정황하환응고필표기지간적관계。의재선택호위협동작용우세기인형적전제하,부집진가능다적SNP표기。
Ornithine decarboxylase (ODC), arate-limiting enzyme in polyamine synthesis,is involved in several bio-logical processes. PCR-RFLP was established to detect genotypes of ten SNPs (six for jlODC1a and four for jlODC1b) in about 900 individuals from 12 Cyprinus carpio var. jian families. The minimum allele frequency (MAF) was 0.14—0.48. The correlation between the genotypes of ten SNPs and body weight gain indicated that there are seven SNP loci in the female and four SNP loci in the male , and that the body weight gain is associated with the quantity of the SNPs. The individuals with four markers grew obviously faster than those with 0—3 markers, and grew 14%faster than those with 0 markers, which also reflected growth as a quantitative trait. The diplotype analysis of the screened SNPs demonstrated that individuals with four heterozygous advantage genotypes 4567 (XXXXXXACCTCTCT) were the fas-test growth ones that were 26.6%faster than 0 advantage genotype individuals, which could be used in the fast-growth jian carp molecular breeding. Moreover, the diplotype analysis explored the exist of either antagonistic or synergistic interaction between different loci, suggesting that the choice of mutually synergistic advantages genotype and as much as possible SNP markers could benefit the future breeding programs.