CAJ | 학술논문

为获知微颗粒饲料中添加精胺对半滑舌鳎仔稚鱼肠道发育的影响,试验以添加0,0.10%,0.33%精胺的微颗粒饲料(F1、F2、F3)和活饵料卤虫(F4)饲喂初始体长为2 cm左右的半滑舌鳎稚鱼。养殖28d后结果表明,卤虫组(F4)的特定生长率最高,饲料组中F2组特定生长率要显著高于F1和F3组(P<0.05)。F3组的存活率仅为60.27%,显著低于其他各组(P<0.05)。消化酶活力测定结果中, F2组在14d和28d时都含有较高的碱性磷酸酶比活力和亮氨酸氨基肽酶比活力,较低的亮氨酸-丙氨酸肽酶比活力。卤虫组的肠道发育情况最好,微绒毛长度显著大于其他各组(P<0.05)；黏膜厚度略小于 F2组,但是没有显著性差异(P>0.05)；饲料组中F2组微绒毛长度和黏膜厚度都显著大于F1和F3组(P<0.05)。研究表明,在微颗粒饲料中添加0.10%的精胺(F2组)对半滑舌鳎稚鱼肠道发育有促进作用。
위획지미과립사료중첨가정알대반활설탑자치어장도발육적영향,시험이첨가0,0.10%,0.33%정알적미과립사료(F1、F2、F3)화활이료서충(F4)사위초시체장위2 cm좌우적반활설탑치어。양식28d후결과표명,서충조(F4)적특정생장솔최고,사료조중F2조특정생장솔요현저고우F1화F3조(P<0.05)。F3조적존활솔부위60.27%,현저저우기타각조(P<0.05)。소화매활력측정결과중, F2조재14d화28d시도함유교고적감성린산매비활력화량안산안기태매비활력,교저적량안산-병안산태매비활력。서충조적장도발육정황최호,미융모장도현저대우기타각조(P<0.05)；점막후도략소우 F2조,단시몰유현저성차이(P>0.05)；사료조중F2조미융모장도화점막후도도현저대우F1화F3조(P<0.05)。연구표명,재미과립사료중첨가0.10%적정알(F2조)대반활설탑치어장도발육유촉진작용。
To study the effects of spermine on the intestinal development of Cynoglossus semilaevis postlarvae, we se-lected the subject with initial length of about 2 cm and fed them with microdiets containing 0 (F1), 0.10%(F2), 0.33%(F3) of spermine and live Artemia (F4) for 28 days. The results showed that F4 group exhibited the highest growth and survival rates. The specific growth rate of F2 group was significantly higher than that of F1 and F3 groups (P<0.05). The survival rate of F3 group was 60.27%, which was significantly lower than that of other groups (P<0.05). At day 14, the alkaline phasphatase relative activities (AP) in the bodies of fish fed with spermine-containing microdiet were sig-nificantly higher than that in fish fed with the Artemia (P<0.05);there was no significant difference among groups fed with spermine-containg mecrodiets (P>0.05). At day 28, the AP of fish fed with 0.10% spermine was significantly higher than other groups (P<0.05);no significant difference was found among other groups (P>0.05). F2 and F3 groups showed higher leucine aminopeptidase (LAP) specific activities than F1 and F4 groups at day 14 and 28 (P<0.05), but there was no significant difference between F2 and F3 (P>0.05). F2 group showed the lowest leucine-alanine peptidase (leuala) specific activity at day 14 and 28 (P<0.05). The microvilli length of Artemia group was significantly greater than that of the other groups (P<0.05). The mucosal layer of Artemia group was apparently but insignificantly hinner than that of F2 group (P>0.05). Both the length of microvilli and the thickness of the mucosal layer in F2 were greater than those of F1 and F3 groups (P<0.05). These results suggested that 0.10%spermine in microdiet had positive effects on the intestinal development of tongue sole postlarvae.